虎耳草的化学成分及生物活性研究

Chemical Constituents of Saxifraga stolonifera(L.)Meerb.and Biological Activity

从虎耳草分离得到8个单体化合物,运用MS、IR、1H NMR和13C NMR等波谱解析方法并结合文献对照分别鉴定为:原儿茶酸(1),琥珀酸(2),桦木酸(3),原儿茶酸甲酯(4),5,7-二羟基色原酮(5),槲皮素3-O-β-L-鼠李糖苷(6),槲皮素5-O-β-D-葡萄糖苷(7),1-O-β-D-glucopyranosyl-(2S,3S,4R,8E/Z)-2-[(2'R)-2'-hydroxytetracosanoylamino)]-8-octadecene-1,3,4-triol(8)。除化合物1,2,7之外,其它化合物均为首次从虎耳草属植物中分离得到。以MTT法测试化合物对PC-3前列腺癌细胞的体外抑制作用,以平板打孔法测试提取浸膏及化合物对不同菌种的抑制作用。结果表明化合物6,7对PC-3前列腺癌细胞的生长有一定的抑制作用,化合物2对枯草芽孢杆菌有潜在的抑制作用。

Eight compounds were isolated from Saxifraga stolonifera （L.） Meerb. The compounds were identified by spectral analysis （ MS,IR, t H NMR and 13 C NMR） as 3,4-dihydroxy benzoic acid （ 1 ）, amber acid （2） , bettflinic acid （3）, 3,4 -Dihydroxybenzoic acid methyl ester （ 4 ）, 5,7-Dthydroxyehromone （ 5 ）, Quercetin 3-O-β-L-rhamnopyranoside （6） ,Quercetin 5-O-β-D-glucopyranoside （7） ,1-O-fl-D-glucopyranosyl-（2S,3S,4R,8E/Z）-2-[ （2＇R）-2＇-hydroxytetra- cosanoylamino） ] -8-octadecene-1,3,4-triol （8）. In addition to compounds 1,2,7, other compounds were first isolated from Saxifi＇aga stolonifera （L.） Meerb. Testing the compounds on PC-3 prostate cancer cells in vitro inhibition by Mq＇T showed that compounds 6 and 7 had the certain inhibition to the PC-3 prostate anceer,the extracts and compounds were tested by plate diffusion method for the inhibitory effect of different strains, compound 2 on Bacillus subtilis had potential inhibition.