淫羊藿醇提物对内皮细胞释放NO的影响

Effects of Alcohol Extract of Herba Epimedii on the Release of NO from Human Umbilical Vein Endothelial Cells in Vitro

为了了解淫羊藿治疗阳痿的作用机制 ,采用Geiess试剂检测淫羊藿醇提物及灌服淫羊藿醇提物小鼠血清对内皮细胞释放NO的影响。结果显示 :淫羊藿醇提物直接加入内皮细胞培养液中不影响NO产生 ,1 2 0min ,1 80min采集的含淫羊藿醇提物血清能促进NO的产生 ,效应高峰在 1 2 0min。结果提示 :促进内皮细胞释放NO可能是淫羊藿治疗阳痿的作用机制之一。

To investigate the mechanism of Herba Epimedii alcohol extract for impotence, Giess reagent was used to observe the effect of the alcohol extract of Herba Epimedii in mice and its effect on release of NO from human umbilical vein endothelial cells in vitro was also studied. The results showed that alcohol extract of Herba Epimdii had no effect on the release of NO. But the serum collected from the mice after the medication of alcohol extract of Herba Epimedii for 120min and 180min promoted the production of NO, and the reaction reached the peak at 120 min. It is indicated that the promotion of NO release from endothelial cells may be one of the mechanisms of Herba Epimedii for impotence.