扶正祛邪丹对K562细胞株及造血干细胞的影响

Experimental study on K562 cells and hematopietic stemcells with Fuzheng-Quxie Tablet

目的 :研究扶正祛邪丹对 K562细胞株和小鼠造血干细胞的影响 ,探讨扶正祛邪丹治疗骨髓增生异常综合征的疗效机理。方法 :扶正祛邪丹含药兔血清培养 K562细胞株 ,用流式细胞仪检测 P53蛋白表达、细胞活力、细胞凋亡与细胞周期 ;用造血干细胞培养法观察扶正祛邪丹对 BALB/C小鼠骨髓造血干细胞增殖的影响。结果 :扶正祛邪丹对 K562细胞株 P53蛋白表达有抑制作用 ,对红系造血祖细胞有促进作用。不能诱导细胞凋亡 ,对细胞增殖周期无明显影响 ,对小鼠骨髓粒系造血干细胞无明显促进作用。结论 :扶正祛邪丹促进红系造血干细胞增殖 ,降低 P53蛋白表达 ,可能为其主要疗效机理。

Objective:To study the mechanism of Fuzheng Quxie Tablet (FZQXD) in treating myelodysplastic syndromes (MDS).Methods: The in vitro effect of FZQXD on K562 cell line and hematopietic stem cells were studied with flow cytometery and cell cultuer.Results:FZQXD inhibited P 53 protein expression,markedly promoted the proliferation of colony forming unit erythrocytes(CFU E).no effect on K562 cell in inducing apoptosis and cell cycle.Conclusions:That FZQXD slightly inhibited P 53 protein expression and markedly promoted the proliferation of colony forming unit erythrocytes (CFU E) might be one of the mechanisms in treating myelodysplastic syndromes.