高效液相色谱法同时测定调味品及肉制品中10种偶氮染料

Simultaneous determination of ten azo dyes in condiments and meat products by high performance liquid chromatography

目的建立调味品及肉制品中10种偶氮染料(红2 G、罗丹明B、酸性橙II、苏丹红I^IV、碱性橙II、碱性橙21、碱性橙22)的快速高效液相色谱检测方法。方法样品经乙腈超声提取,液相色谱分离,外标法定量。使用Thermo BetaBasic-18(4.6 nm×250 nm,5μm)色谱柱,以乙腈、20 mmol/L乙酸铵水溶液和四氢呋喃为流动相进行梯度洗脱,紫外检测波长分别为414 nm(酸性橙II)、500 nm(苏丹红I^IV、碱性橙II、碱性橙21、碱性橙22)、540 nm(红2 G、罗丹明B)。结果 10种偶氮染料的分离效果良好,在0.25μg/ml^20.0μg/ml范围内具有良好的线性关系(相关系数均大于0.9992),相对标准偏差(n=6)均小于7.3%。在3个添加水平(1 mg/kg、5 mg/kg、10 mg/kg)下平均回收率为73.8%~107.3%。结论该方法快捷、准确、操作成本低,适用于食品中非法添加的多种工业染料的同时测定。

Objective A method was developed for the simultaneous determination of 10 azo dyes （red 2G, rhodamine B, or- ange II sodium salt, Sudan I - IV, ehrysoidine, astrazon orange G, astrazon orange R） in condiments and meat products using high performance liquid chromatography. Methods Samples were ultrasonically extracted with acetortitrile, separated by liquid chromatography, then quantified by external standard method. The target analytes were separated at flow rate of 1.0 ml/min on Thermo BetaBasic -18（4.6 nm ×250 nm,5 μm） column for gradient elution. The mobile phase was acetonitrile, 20 mmol/L ammonium acetate and tetrahydrofuran. The wavelengths for detection were 414 nm（ orange II）, 500 nm（ Sudan I - IV, chry- soidine, astrazon orange G, astrazon orange R）and 540nm（red 2 G, rhodamine B）. Results The results showed that the 10 azo dyes can be separated efficiently. This method showed a good linearity in the range of 0.25 μg/ml - 20.0μg/ml with the correlation coefficients （r） greater than 0. 9992. The relative standard deviations （n = 6） were less than 7.3%. The average recoveries of the ten azo dyes at three levels （ 1 mg/kg, 5 mg/kg and 10 mg/kg） ranged from 73.8% to 107.3%. Conclusion This method, owing to its rapidity, accuracy and low operation costs, is suitable for the simultaneous determination of illegally added industrial synthetic dyes in foods.