摘要
目的建立中药材中黄曲霉毒素G2、G1、B2、B1的UHPLC-MS/MS测定方法。方法以多种中药材为研究基质,采用甲醇水系统提取中药材中的黄曲霉毒素G2、G1、B2、B1,再通过免疫亲合柱净化除杂,用超高效液相色谱-三重四级杆串联质谱进行分析测定。结果黄曲霉毒素G2、B2在0.045 ng/ml^3 ng/ml范围内与峰面积呈良好的线性关系,黄曲霉毒素G1、B1在0.15 ng/ml^10 ng/ml范围内与峰面积呈良好的线性关系,r>0.999。多种中药材的回收率在63%~118%之间。结论本法灵敏、快速、准确、专属性强,可用于多种中药材中黄曲霉毒素G2、G1、B2、B1的测定。
Objective To establish a UHPLC - MS/MS method for determination of aflatoxin G2, G1, B2, B1 in Chinese herbs. Methods The aflatoxins G2, G1 , B2 , B1 in several Chinese herbs were extracted by 70% methanol, purified by immu- noaffinity column, and then analyzed by UHPLC -triple quadrupole MS. Results There was a good linear relationship within the range of 0.045 ng/ml - 3 ng/ml for aflatoxin G2, B2, and 0.15 ng/ml - 10 ng/ml for aflatoxin G1 , B1 （ r 〉 0. 999 ）. The recovery were between 63% - 118%. Conclusion The method is sensitive, rapid, accurate and specific enough to determine aflatoxin G2, GI, B2, B1in Chinese herbs.
出处
《中国卫生检验杂志》
北大核心
2014年第2期190-193,共4页
Chinese Journal of Health Laboratory Technology
