A lzheimer病患者血清IL-2、sIL-2R水平的研究

Interleukin-2 and Soluble Interleukin-2 Recepter in Serum of Patients with Alzheimer Disease

目的 :Alzheimer病 (AD)是由多种病因引起的涉及多种病理机制和出现多种病理表现的多因素性疾病。近年来研究发现 AD患者血清中细胞因子水平增高以及皮质、海马内神经炎性斑数量增加 ,表明免疫炎症机制在 AD的发生、发展中起重要作用。本研究拟通过对 AD患者血清 IL - 2、 s IL - 2 R水平的检测 ,来探讨其在 AD慢性炎症病理过程中的作用。方法 :采用双抗体酶联免疫吸附试验 (EL ISA) ,检测 10例 AD患者血清 IL 2及 s IL 2 R水平 ,并与血管性痴呆 (VD)组及正常对照组做了比较。结果 :AD组血清中 IL- 2水平为 35 2± 33.4pg/ ml,明显高于 VD组 (2 83.6± 6 2 .9pg/ ml)和正常对照组 (2 5 8.5± 49.1pg/ ml) ,差异显著 (P<0 .0 0 5 ) ;AD组 s IL - 2 R水平为 81± 37.3pmol/ L ,明显高于VD组 (5 4.1± 30 .9pmol/ L )和正常对照组 (48.3± 18.3pmol/ L ) ,差异显著 (P<0 .0 5 )。结论 :说明 AD患者脑内免疫细胞被激活 ,IL- 2、s IL- 2 R参与了 AD的慢性炎症改变过程。血清 IL- 2和 s IL- 2 R可作为检测 AD外周血的免疫标记物。

Objective: Alzheimer's disease has been took on multi pathogenesis and displayed multi pathologic changes. Lately research indicated increased cytokine levels in serum and cerebrospinal fluid and elevated neuritic plaques in cortices and hippocampus, these results suggest that inflammatory and immune mechanisms contribute a important role in the occurrence and development of Alzheimer's disease. In this study, we investigated the functions of IL 2 and sIL 2R in the chronic pathologic process in AD patients. Methods: Using enzyme linked immunosorbent assays (ELISA), we quantitated the levels of IL 2 ands IL 2R of patients with Alzheimer's disease (n=10), vascular dementia and control subjects. Results:AD patients showed significantly increased concentrations of IL 2 both in serum (AD=352±33.4pg/ml, VD=283.6±62.9pg/ml,C=258.5±49.1pg/ml), P<0.005; while the concentrations of sIL 2R were also significantly elevated in AD patients compared with VD patients and control subjects in serum (AD=81±37.3pmol/l, VD=54.1±30.9pmol/L, C=48.3±18.3pmol/L), P<0.005.Conclusion: IL 2 and sIL 2R take part in the inflammatory progress of AD Enhanced serum levels of IL 2 and sIL 2R indicate that these two parameters can be seemed as neuroimmunc markers of AD patients in the peripheral blood.