水稻落粒性的遗传分析和基因定位

Genetic Analysis and Molecular Mapping of Seed Shattering in Rice

利用爪哇稻NS2098与籼稻桂农占杂交并自交获得的F2群体,对水稻落粒性进行了遗传分析和基因定位。结果表明,难落粒性状受1对显性基因Sh(t)控制。利用分子标记将sh(t)定位在第1号染色体的SSR标记RM11869、RM3825、RM11875、RM8278、RM315、RM11893和RM1387附近,与它们之间的遗传距离分别是1.65,1.59,1.46,1.65,1.72,1.72和4.13 cM,与落粒性基因qSH1的1个SNP临近。序列分析表明,NS2098在该SNP位点为碱基T,而桂农占为碱基G,与qSH1基因的报道结果一致,推测Sh(t)与qSH1可能是同一基因。

The genetic analysis and gene mapping of seed shattering in rice were conducted using F1 and F2 popula- tions of the cross of NS2098, a nonshattering javanica mutant, with Guinongzhan, a shattering indica variety. The results indicated that nonshattering was controlled by a single dominant nuclear gene, tentatively named Sh （t）. U- sing simple sequence repeat （SSR） markers, the sh （t） gene was mapped around the markers of RMl1869, RM3825, RMl1875, RM8278, RM315, RMl1893 and RM1387 on Chromosome 1, with a genetic distance of 1.65, 1.59, 1.46, 1.65, 1.72, 1.72 and 4.13 cM, respectively, and it was very close to the locus of an SNP of the nonshattering gene qSHI. The sequence analysis showed that the nueleotide of NS2098 was T at the SNP posi- tion while Guinongzhan was G at the SNP position, which is consistent with the previous report of the qSH1 gene. Therefore, it can be inferred that Sh（t） and qSH1 might be the same gene.