CBLB502对放射性肺炎和肺纤维化的防护作用研究

CBLB502 Protects mice radiation pneumonitis and pulmonary fibrosis

目的研究CBLB502对放射性肺炎和放射性肺纤维化的防护作用,从而证实CBLB502作为临床抗放射药物的可行性。方法 C57BL/6J小鼠20 Gy单次照射后24 h、1个月、3个月和5个月取肺组织用TUNEL方法对肺泡上皮细胞和血管内皮细胞的凋亡进行检测,HE染色观察纤维化病理变化,免疫组织化学方法检测特异性指标表达,以及受照部位皮毛和皮肤的病理变化。结果 CBLB502抑制了照射后小鼠肺泡上皮细胞和血管内皮细胞的凋亡,减缓了肺纤维化进程,同时减少层黏连蛋白表达,维持表面活性蛋白B表达量,受照皮肤的炎症反应也明显减轻。结论 CBLB502对照射后放射性肺炎和放射性肺纤维化的发生和进程有防护作用。

Objective To investigate the protective effect of CBLBS02 on radiation pneumonitis and pulmonary fibrosis for confirming the feasibility of CBLB502 as a clinical anti-radiation drug release. Methods With a single 20 Gy ilTadia- tion, C57BL/6J mice was sacrificed on 24 h, 1 month, 3 months and 5 months and lung tissue was assayed by TUNEL method for apoptosis of alveolar epithelial cells and endothelial cells, HE staining showing fibrosis changes, immunohisto- chemistry detecting the expression of specific indicators, as well as pathological changes of the fur and skin radiated site. Results CBLB502 inhibits apoptosis in mice alveolar epithelial cells and vascular endothelial cells after irradiation, slowing the process of pulmonary fibrosis, while reducing the expression of laminin and maintaining the expression of surfac- tant protein B, and the skin inflammation also significantly reduced. Conclusion CBLB502 could alleviate the occurrence of radiation pneumonitis and pulmonary fibrosis as well as radiation-induced skin injury.