响应面法优化狍茸中活性成分提取工艺

Optimization of extraction condition of the activity ingredient from roe deer antler by response surface methodology

目的:优化狍茸活性成分提取工艺。方法:以狍茸为原料,利用乙醇为溶剂对狍茸中活性成分进行提取,在单因素的实验基础上,选取乙醇浓度、提取时间、提取温度为自变量,醇溶物的提取率为响应值,采用Box-Behnken中心组合设计和响应面分析法对其提取工艺进行研究。结果:建立了回归方程的预测模型,在固液比为1:8时,确定最佳提取条件为:乙醇浓度70%、时间2.5 h、温度55℃,此条件下醇溶物的提取率为39.87 mg/g。结论:中心组合设计-响应面法可在连续范围内进行分析,具有实验周期短、精密度高的优点,优化结果可为狍茸产品的开发提供参考依据。

Objective： To optimization extraction-technology in the activity ingredient from roe deer antler. Methods： Roe deer antler for raw materials, uses alcohol for solvent on its in the activity components for extraction, based in single factors of experimental, select alcohol concentration, extraction time, and extraction temperature for variable, alcohol dissolved real of extraction rate for response value, used Box- Behnken center combination design and response surface analysis law, research variable and interactive role on extraction rate of effect. Results： The regression equation prediction models were developed, in solid-liquid ratio is 1：8, determine the best extract conditions： the concentration of alcohol is 70%, time 2.5 h, temperature of 55 ℃, this alcohol-soluble under the conditions of extraction rate of 39.87 mg/ g. Conclusion： Box-Behnken center combination design and method of response surface analysis can analyze in continuity circumscription, it possess forte that test cycle is short and degree of precision is better, the optimization result provide evidence for developing roe deer product.