摘要
建立实时荧光PCR检测丙型副伤寒沙门氏菌(S.Paratyphi C/Salmonella paratyphi C)和猪霍乱沙门氏菌(S.Choleraesuis/Salmonella Choleraesuis)的方法。根据GenBank公布的丙型副伤寒沙门菌和猪霍乱沙门氏菌序列,分别设计引物和Taqman探针,使用168株不同血清型的沙门氏菌和21株变形杆菌等非沙门氏菌进行实时荧光PCR检测的特异性试验,可以实现特异性检测丙型副伤寒沙门氏菌,并且可以同时检测丙型副伤寒沙门氏菌和猪霍乱沙门氏菌。经模拟污染样品检测,灵敏度可达到2～5 cfu/mL的添加浓度。该方法可以快速检测食品中丙型副伤寒沙门菌和猪霍乱沙门氏菌。
The method of real-time polymerase chain reaction（PCR） for detection of Salmonella paratyphi C and Salmonella Choleraesuis was established in this study. Based on conserved sequence of Salmonella paratyphi C and Salmonella Choleraesuis in GenBank, PCR primer and Taqman probe sequences were designed, 168 strains of Salmonella and 21 strains of non-Salmonella, such as Proteus, etc, were detected and identified by the real-time PCR method, the results showed that it was a highly specific identification method for detection of Salmonella paratyphi C, and Salmonella paratyphi C and Salmonella Choleraesuis could be detected simultaneously. The result of detecting simulation samples by this method showed the sensitivity could reach 2～5 cfu/mL. It was a rapid and effective detection method of Salmonella paratyphi C and Salmonella Choleraesuis in food.
出处
《食品科技》
CAS
北大核心
2014年第2期297-301,共5页
Food Science and Technology
基金
辽宁省自然科学基金项目(20102080)
国家科技支撑计划项目(2011BAK10B02)
