摘要
用一张具有 182个 RFL P标记的分子连锁图谱和一套重组自交系 ( RIL)群体 ,对水稻植株主茎总叶片数、叶片生长速率、抽穗期和株高等数量性状进行 QTL区间作图研究 ,定位了影响水稻出叶速率的 8个 QTL ,主茎总叶片数的 2个QTL、抽穗期的 3个 QTL和株高的 4个 QTL。对这些 QTL的遗传效应分析并结合先前用同组合材料研究结果 ,为进一步明确一些 QTL的基因功能提供了有用的信息。如控制水稻主茎总叶片数的一个主效 QTL ,即 QL n3,它同时影响分蘖期出叶速率、抽穗期及株高等数量性状。该位点来自特青的等位基因 ,其加性效应可使水稻在温室冬季短日条件下主茎总叶片数增加1.5叶左右 ,抽穗期延迟 9d,同时具有使分蘖期出叶速率降低 0 .2叶 / 10 d的效应。由于该 QTL位点的基因不受光照长度的影响 ,说明它们有可能是由一个影响水稻基本营养生长期的基因控制或者这些基因紧密连锁 ;而另一个 QTL( QH d8)位点的基因对主茎总叶数。
Quantitative trait loci (QTLs) affecting total leaf number of the main stem, leafing rate at two different growth stages, heading date, and plant height in a recombinant inbred lines (RIL)population from the cross Lemont (japonica)×Teqing (indica) were investigated using a complete RFLP map with 182 marker loci. Eight QTLs for rate of leaf growth at two different stages, two QTLs for total leaf number of main stem, three QTLs for heading date, and four QTLs for plant height were mapped. Combined with previous studies on this cross, these results provided useful information on the nature of some detected QTLs. For example, QLn3, a main effect QTL from Teqing that give 1.5 leaf more of total leaf number, 9 days delayed heading, and 0.2 leaf increase each ten days during tillering stage in greenhouse condition was identified on chromosome 3 near the marker RG348. This QTL appeared to affect the basic vegetative period of rice plants since it was not affected by the daylength. On the other hand, QHd8 which affects heading date, plant height, and total leaf number of main stem appeared to be influenced by daylength.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2001年第1期7-12,共6页
Chinese Journal of Rice Science
基金
国家自然科学基金重点项目
国家重点基础研究发展规划项目! (973项目 )
美国洛克菲勒基金资助项目
关键词
重组自交系
出叶速率
主茎总叶数
基因定位
水稻
recombinant inbred line
leafing rate
total leaf number
gene location
rice