摘要
An emerging green solvent called a deep eutectic solvent(DES) was applied to the extraction and determination of catechin(C),(+)epicatechin gailate(ECG) and (-)epigallocatechin gallate(EGCG) from Chinese green tea.After evaluating different combinations of them by extraction methods and DESs,a DES-based extraction method was established and optimized by a systematic investigation of the influencing factors.As a result,a total of 3.629,35.25 or 114.2 mg/g catechin,(+)epicatechin gallate or (-)epigaliocatechin gallate were extracted respectively under optimal conditions with extraction efficiencies of 82.7%,92.3% and 97.0%,respectively.By comparing with other common used solvents for extracting catechin compounds,DESs were proved to be potential extraction solvents for bioactive ingredients.
An emerging green solvent called a deep eutectic solvent(DES) was applied to the extraction and determination of catechin(C),(+)epicatechin gailate(ECG) and (-)epigallocatechin gallate(EGCG) from Chinese green tea.After evaluating different combinations of them by extraction methods and DESs,a DES-based extraction method was established and optimized by a systematic investigation of the influencing factors.As a result,a total of 3.629,35.25 or 114.2 mg/g catechin,(+)epicatechin gallate or (-)epigaliocatechin gallate were extracted respectively under optimal conditions with extraction efficiencies of 82.7%,92.3% and 97.0%,respectively.By comparing with other common used solvents for extracting catechin compounds,DESs were proved to be potential extraction solvents for bioactive ingredients.