外周血循环MUCl mRNA表达水平与乳腺癌、肺癌的相关性研究

Correlation study of the MUC1 mRNA expression in peripheral blood with breast cancer and lung cancer

目的研究外周血循环MUClmRNA表达水平与乳腺癌、肺癌、其他癌等疾病的相关性以及对乳腺癌等疾病的诊断价值。方法分别采集健康对照组、良性肿瘤组、乳腺癌组、肺癌组以及其他癌组入院患者的血清样本，采用实时定量荧光逆转录聚合酶链反应（RT-PCR）方法检测外周血循环MUClmRNA含量。结果良性肿瘤组外周血循环MUClmRNA表达水平及阳性率（10％）与健康对照组比较差异无统计学意义（P〉0．05），乳腺癌组外周血循环MUClmRNA表达水平及阳性率（50％）与健康对照组比较差异有统计学意义（P〈0．01），乳腺癌组中以浸润性导管癌最高，各种类型乳腺癌比较差异无统计学意义（P〉0．05），肺癌组外周血循环MUClmRNA表达水平及阳性率（60％）与健康对照组比较差异有统计学意义（P〈0．01），其他癌组外周血循环MUClmRNA表达水平及阳性率（20％）与健康对照组比较差异无统计学意义（P〉0．05）。结论MUCImRNA可以作为乳腺癌、肺癌的诊断标志物，但对乳腺癌分型的诊断没有价值。

Objective To study the correlation of expression of MUC1 mRNA in peripheral blood with breast cancer and lung cancer, and its diagnostic value on breast cancer. Methods The ser- um in health control group, benign tumor, breast cancer, lung cancer and other cancer groups was col- lected, then the expression of MUC1 mRNA was detected by reverse transcriptase polymerase chain reac- tion （RT-PCR）. Results MUC1 mRNA expression and positive rate （ 10% ） in benign tumor group had not statistically significant correlation with healthy control group （ P 〉 0.05 ）. The expression and positive rate （50%） in breast cancer group had significant difference with healthy control group （ P 〉 0.01 ）, MUC1 mRNA expression of infiltrating ductal carcinoma of breast was the highest in this group. Various types of breast cancer had no significant difference （P 〉0.05）. The expression and positive rate （60%） in lung cancer group had significant difference with healthy control group（ P 〉 0.01 ） , and in other cancer group had no significant difference（P 〉 0.05）. Conclusions MUC1 mRNA can be the marker to diag- nose breast cancer and lung cancer, but it has no value on diagnosis of breast cancer classification.