滇杨一年生扦插苗侧芽内源激素含量的变化分析

Analysis on the Contents Changes of Endogenous Hormones in Lateral Buds of One-year Old Cuttings of Populus yunnanensis

以滇杨优树无性系1a生扦插苗为试材,采用酶联免疫吸附法(ELISA),研究测定了滇杨侧芽在落叶期(10月)、休眠期(1月)、萌动期(3月)脱落酸(ABA)、生长素(IAA)、玉米素(ZR)含量的动态变化规律。结果表明:滇杨1a生扦插苗ABA和IAA含量在3个时期的变化趋势相同,均呈逐步上升趋势,而ZR含量呈先上升后下降趋势,在休眠期达到最高水平,3种激素的含量在不同时期之间均具有极显著差异;ZR/ABA、ZR/IAA和ZR/(ABA+IAA)比值变化规律一致,呈近似抛物线趋势,在休眠期呈现最高峰,且极显著高于落叶期和萌动期。

Taking one-year old cuttings of Populus yunnanensis as material, the dynamic changes of ABA, IAA, ZR contents in lateral bud at defoliation stage （October）, dormancy stage （January） and germination stage （March） were studied using enzyme linked irnmunosorbent assay （ELISA）. The results showed that the ABA and IAA content present the same trends at three growth stages, both of which showed an escalation trend. While the ZR content rised at first and fell after that, which achieved the highest level at dormancy stage. Three kinds of endogenous hormones had highly significant differences among different stages. The variation of ZR/ABA, ZR/IM, ZR/（ABA ＋ 1M） ratios were accordance, which present the parabolic trend and arrived the highest peak at dormancy stage, as well as significantly higher than defoliation stage and germination stage.