药用裸花紫珠微繁技术增殖培养基筛选研究

Screening Study on Proliferation Medium of Micropropagation Technique for Callicarpa nudiflora

以裸花紫珠丛生芽为外植体,在光照时间9h/d,光照强度1 500lx,温度(26±2)℃,pH 5.8的培养条件下,研究比较了不同无机盐量MS培养基、不同植物生长调节剂种类及配比、蔗糖浓度、培养周期等因素对裸花紫珠生长状况的影响,以期筛选出裸花紫珠微繁技术最佳增殖培养基。结果表明:丛生芽继代增殖的最佳配方为:MS+6-BA 2.0mg/L+NAA 0.05mg/L+蔗糖30g/L,30d的增殖系数达到10以上,丛生芽长势好。

Taking the clustered buds of Callicarpa nudiflora as the explant, the effect of inorganic salt types and concentrations of plant growth regulators, sucrose concentration.culture cycle and other factors on growth of Callicarpa nudiflora were studied under the conditions as light time 9 h/d,light intensity of 1500 lx, temperature of （26±2）℃ and pH 5. 8,in order to screen the optimum proliferation medium of rnicropropagation technique. The results showed that the best formula for clustered buds subculture proliferation was MS ＋ 6 - BA 2.0 mg/L ＋ NAA 0.05 mg/L ＋ sucrose 30 g/L, proliferation coefficient was over 10 in 30 d and clustered buds growth were potential.