459例女性健康体检者HPV基因型及高危因素分析

The Analysis of HPV Genotypes and Related High Risk Factors in 459 Women of Annual Health Examination

目的研究459例女性健康体检者宫颈人乳头瘤病毒(HPV)感染情况、基因型别分布及其高危因素。方法采用巢式PCR和焦磷酸测序技术检测459例受检者宫颈脱落细胞中HPV分型情况,同时收集受检者一般资料、月经婚育史、生殖道疾病既往史,分析HPV感染的相关危险因素。结果 HPV阳性检出率为17.9%,共检测出7种高危基因型别,其中HPV16检出率最高(9.8%),其次是HPV58(7.0%),HPV18(5.2%)。单纯感染、双重感染、多重感染的检出率分别是9.6%、4.8%、1.5%。饮酒、经济收入<3 000元/月、性伴侣>1个、性生活频率>4次/月、伴有宫颈糜烂是HPV感染的危险因素(P<0.05)。结论 HPV DNA基因型别检测可为宫颈癌的早期筛查和HPV疫苗的研发使用提供重要信息,为高危人群的早预防、早诊断、早治疗提供重要依据。

Objective To investigate the cervical human papillomavirus （HPV） infection, genotypes and related high risk factors in 459 women of annual health examination. Methods A total of 459 cervical cytology specimens were de-tected with nest PCR and pyrosequencing methods. Questionnaires were collected simultaneously and the risk factors of HPV infection were analyzed. Results The detection rate of HPV-positive samples was 17.9%. Seven kinds of high-risk genotypes of HPV were found. The detection of HPV 16 was the most common （9.8%）, followed by HPV58 （7.0%） and HPV18 （5.2%）. The detection rates of simplex infection, dual infections and multiple infections were 9.6%, 4.8%and 1.5%respectively. The risk factors for HPV infection included alcohol consumption,income 〈3 000 yuan/ month, sexual part-ners＆gt;1, frequency of sexual activity〈4 times/month and cervical erosion （P〈0.05）. Conclusion HPV DNA genotyping can provide important reference for HPV screening at early time and the application of HPV vaccines, which also provide sig-nificant evidence for the prevention, diagnosis and treatment of cervical cancer at early stage for the high risk population.