宫颈病变中hTERT基因扩增与高危型HPV感染的关系

Association between hTERT Gene Amplification and High-Risk HPV Infection in Cervical Lesions

目的探讨人端粒酶逆转录酶(hTERT)基因扩增和高危型人乳头瘤病毒(HR-HPV)感染在宫颈病变中的关系。方法获取34例正常人,31例宫颈上皮内瘤变(CIN)Ⅰ级,33例CINⅡ级,34例CINⅢ级,20例宫颈癌患者的宫颈脱落细胞标本,应用聚合酶链反应(PCR)-反向杂交芯片法检测HPV DNA阳性情况,应用荧光原位杂交技术(FISH)检测hTERT基因扩增情况。结果共检出20种HR-HPV:HPV16,18,26,31,33,35,39,45,51,52,53,56,58,59,66,67,68,69,73,82。CINⅡ级(72.73%)、CINⅢ级(85.29%)、宫颈癌组(90.00%)的HR-HPV DNA阳性比例高于对照组(20.59%),对照组与CINⅠ级(54.84%)组比较差异无统计学意义。宫颈癌组(80.00%)hTERT基因扩增的阳性率高于对照组(0)、CINⅠ级(3.22%)、CINⅡ级(18.18%),宫颈癌与CINⅢ级组(41.18%)比较差异无统计学意义。hTERT基因扩增与HR-HPV感染呈正相关(r=0.238,P<0.05)。结论宫颈病变中hTERT基因扩增与HR-HPV感染呈正相关,HR-HPV感染可能是hTERT基因异常扩增的早期事件,hTERT基因检测可能作为宫颈癌前病变监测的辅助指标,应用于临床早期宫颈病变的诊断中。

Objective To investigate the relationship between the amplification of human telomerase reverse tran-scriptase （hTERT） gene and high-risk human papillomavirus （HR-HPV） infections in cervical intraepithelial neoplasia （CIN） and cervical carcinoma. Methods The cervical epithelial cells were collected from 34 samples of normal cervical epithelium, 31 samples of CIN （gradeⅠ）, 33 samples of CIN （gradeⅡ）, 34 samples of CIN （gradeⅢ） and 20 samples of cer-vical carcinoma. HPV DNA was detected by polymerase chain reaction-reverse dot blot hybridization （PCR-RDB） and the amplification of hTERT gene was detected by fluorescence in situ hybridization （FISH）. Results Twenty subtypes of HR-HPV were detected including HPV16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 67, 68, 69, 73 and 82. The inci-dence of HR-HPV infection was higher in CINⅡgroup （72.73%）, CINⅢgroup （85.29%） and cervical carcinoma group （90.00%） than that of normal cervical epithelium group （20.59%）. There was no significant difference in the positive rate of HR-HPV DNA between CINⅠ group （54.84%） and normal cervical epithelium group （P 〈 0.005）. The positive rate of hTERT gene amplification was higher in cervical carcinoma group （80.00%） than that of normal cervical epithelium group （0）. There were no significant differences in the positive rates of hTERT gene amplification between CINⅠgroup （ 3.22%）, CIN Ⅱ group （18.18%）, cervical carcinoma group and CIN Ⅲ group （41.18%）. There was positive correlation between hTERT gene amplification and HR-HPV infection （r=0.238, P〈0.05）. Conclusion The incidence of HR-HPV infection was positively correlated with hTERT gene amplification in cervical lesions. HR-HPV infection may be an early event of ab-normal amplification of hTERT gene. The detection of HPV-DNA and hTERT gene can be used in the clinical diagnosis of early cervical lesions.