乙酰肝素酶、蛋白激酶CK2β与缺氧诱导因子-1α在鼻咽癌组织中的表达及其相关性研究

Expressions and correlation of HPA,CK2β and HIF-1α in nasopharyngeal carcinoma

目的:检测乙酰肝素酶(HPA)、蛋白激酶CK2β(CK2β)、缺氧诱导因子-1α(HIF-1α)在鼻咽癌(NPC)组织中的表达及其与临床特征之间的关系;并分析三者在NPC组织中表达的相关性。方法:采用免疫组织化学染色技术Super-Vision法检测49例NPC组织标本及15例慢性鼻咽炎(CNT)组织标本中HPA、CK2β及HIF-1α的表达水平。结果:HPA、CK2β与HIF-1α在NPC组织中的表达水平均高于CNT组织中的表达,差异均有统计学意义(P<0.05);HPA、CK2β与HIF-1α的表达与NPC临床分期、治疗后有无复发或转移相关(P<0.05),与患者的性别无关(P>0.05);HIF-1α的表达水平与NPC患者的年龄密切相关(P<0.01),而HPA、CK2β的表达水平则与NPC患者的年龄无关(P>0.05);HPA、CK2β与HIF-1α任意两者间在NPC组织中的表达均呈正相关性(P<0.05)。结论:HPA、CK2β和HIF-1α参与了NPC的发生和发展、浸润及转移;在NPC的发生发展过程中,HPA、CK2β和HIF-1α之间可能存在协同作用。

Abstract Objective：To investigate the expression of HPA,CK213 and HIF-la gene in nasopharyngeal carci- noma（NPC） tissues, and the correlation between their expression with the clinical characteristics of NPC and the relativity of HPA,CK2[3 and HIF-la gene in NPC tissues. Method： HPA, CK213 and HIF-la were detected with Super-Vision immunohistochemical method using antibody in 49 NPC specimens and 30 specimens with chronic na- sopharyngitis tissue（CNT）. Result： The expression of HPA, CK2~ and HIF-Ia in NPC tissue were significantly higher than those in CNT tissue （P^0.05, separately）. The expression of HPA,CK2~3 and HIF-Ic~ were signifi- cantly related to the TNM stage and whether recurrence or metastasis occur after treatment （P＇~0.05, separate- ly）, but there was no obvious correlation between its expression and the sex of NPC patient（P~＇0.05）. The ex- pression of HIF-I~ was significantly related to the age of NPC patient （P^0.05）, while HPA,CK2~ were not. The expression of HPA,CK213 and HIF-lc~ in NPC tissue was positively correlated with each other （P^0.05, sep- arately）. Conclusion： HPA, CK213 and HIF-I~ play synergetic role in development of NPC, which plays an impor- tant role in invasiveness,recurrence and metastasis of NPC. There could be a positive cooperation among HPA, CK213 and HIF-lc~ in the carcinogenesis and development of NPC.