不同海拔高度祁连圆柏叶中挥发性成分的比较

Comparison of volatile components of Sabina przewalskii in different altitudes

为探究祁连圆柏叶中挥发成分与海拔高度的关系,以生长在不同海拔高度的祁连圆柏叶为原料,采用水蒸馏法提取挥发油,运用GC-MS进行化学成分分析。结果表明:5种挥发油共鉴定出50种化合物,5个不同海拔高度的挥发油中分别鉴定出23、28、25、25和22种化合物,各占挥发油总量的91.85%、95.28%、87.17%、86.48%和85.03%。挥发油中单萜及其含氧化合物质量分数随海拔高度的升高而降低,倍半萜及其含氧衍生物的质量分数随海拔高度的上升而呈升高趋势。5种挥发油化学组成各有异同,共同含有的成分有7种,分别为α-蒎烯、1-甲基-4-异丙基-1,4-环己二烯、(R)-(-)-4-萜品醇、大根香叶烯、(+)-δ-杜松烯、榄香醇和柏木脑。海拔高度的变化对祁连圆柏挥叶发油成分及其质量分数有一定规律性的影响。

Variation in the content and composition of Sabina przewalskii essential oils was studied using water distillation followed by GC-MS. Samples of S. przewalskii＇s leaves were harvested from five different altitudes. A total of 50 different compounds in the essential oils were identified. Twenty-three, 28, 25, 25 and 22 compounds in the volatile oils from five different altitudes were identified, respectively, and their percentage compositions were separately 91.85% , 95.28% , 87.17% , 86.48% and 85.03%. The identified components were represented mainly by monoterpenes, sesquiterpenes and their oxygenous derivatives. The relation between altitude and contents of various class compounds was analyzed. The common seven constituents among the essential oils were a-pinene, 1-methyl4-（ 1-methylethyl）-l, 4- cyclohexadiene, 4-methyl-l-（ 1-methylethyl ）-3-cycohexen-l-ol, [ s-（ E, E ） ]-1-methyl-5-methylene-8- （ 1-methylethyl ）-1, 6-cyclodecadiene, （ 1S-cis ）-1, 2, 3, 5, 6, 8a-hexahydro4, 7-di-methyl-l-（ 1- methylethyl）-Naphthalene, [ 1R-（ 1. alpha., 3. alpha., 4. beta. ）]4-ethenyl-. alpha. ,. alpha., 4- trimethyl-3-（1-methylethenyl）-cyclohexanemethanol, Cedrol. The variation of content and composition of S. przewalskii volatile oils was regularly affected by the ecological environment of different altitudes.