双基因活化纳米骨浆的体内成骨

In vivo osteogenesis by implanting dual gene activated nanobone putty

背景:前期实验构建了骨形态发生蛋白2/血管内皮生长因子双基因活化纳米骨浆。目的:观察骨形态发生蛋白2/血管内皮生长因子双基因活化纳米骨浆在动物体内成骨的基因表达和骨形成效果。方法:取昆明小鼠24只,在其中12只右侧大腿后群肌袋内注入骨形态发生蛋白2/血管内皮生长因子+纳米骨浆,左侧大腿后群肌袋内注入空白质粒+纳米骨浆或纳米骨浆;在剩余12只小鼠右侧大腿后群肌袋内注入骨形态发生蛋白2+纳米骨浆,左侧大腿后群肌袋内注入空白质粒+纳米骨浆或纳米骨浆。术后2,4周取材作影像学检查、组织学观察和分子生物学检测。结果与结论:术后各时间点骨形态发生蛋白2/血管内皮生长因子+纳米骨浆组、骨形态发生蛋白2+纳米骨浆组均有骨样组织形成;骨形态发生蛋白2/血管内皮生长因子+纳米骨浆组局部有明显骨形态发生蛋白2和血管内皮生长因子的mRNA表达,并且碱性磷酸酶水平、成骨速度及新生骨量明显优于骨形态发生蛋白2+纳米骨浆组(P<0.05);空白质粒+纳米骨浆组、纳米骨浆组无明显成骨表现。表明纳米骨浆经骨形态发生蛋白2/血管内皮生长因子质粒或骨形态发生蛋白2质粒活化后,在体内具有了一定的成骨能力,且前者在成骨速度和质量方面较后者明显增强。

BACKGROUND： The bone morphogenetic protein 2 （BMP2）/vascular endothelial growth factor （VEGF） dual gene activated nanobone putty has been constructed in the previous experiments. OBJECTIVE： To investigate the effects of osteogenesis and osteogenic gene expression in mice by implanting BMP2/VEGF dual gene activated nanobone putty. METHODS： Twenty-four Kunming mice （48 sides） were randomly divided into four groups. Animals in each group （12 samples） were injected different materials into the right thigh muscle pouches： nanobone putty＋hBMP2/VEGF plasmid; nanobone putty＋hBMP2 plasmid; blank plasmid＋nanobone putty; nanobone putty only. The effects of osteogenesis were evaluated by radiography, histology and molecular biology analysis in 2, 4 weeks after operation. RESULTS AND CONCLUSION： Bone-like tissues were observed in groups of nanobone putty＋hBMP2/VEGF plasmid and nanobone putty＋hBMP2 plasmid after operation. There was apparent BMP2 and VEGF mRNA expression in group of nanobone putty＋hBMP2/VEGF plasmid. Group of nanobone putty＋hBMP2/VEGF plasmid was significantly better than group of nanobone putty＋hBMP2 plasmid in the alkaline phosphatase levels, the speed of osteogenesisas and amount of new bone （P 〈 0.05）. Groups of blank plasmid＋nanobone putty and nanobone putty had no obvious osteogenesis performance. Either BMP2/VEGF dual gene activated nanobone putty or BMP2 gene activated nanobone putty had the osteogenic ability in vivo. And the former was significantly enhanced in the speed and quality of osteogenesis.