黄芪多糖对内毒素血症性肺损伤治疗作用的实验研究

Experimental Study on Effects of Astragalus Polysaccharide on Endotoxemic Lung Injury

目的:探讨中药黄芪多糖(APS)对脂多糖(LPS)诱导的内毒素血症性肺损伤小鼠的治疗作用。方法:40只健康10-12周龄成年C57BL/6J小鼠随机分为正常对照组、肺损伤模型组、地塞米松治疗组和APS治疗组,每组10只。除正常对照组外,其余三组小鼠均采用腹腔注射LPS 5mg/Kg,成功制模。成模后2h、24h和48h,地塞米松治疗组和APS治疗组分别通过腹腔注射地塞米松(5mg/Kg·次)或APS(200mg/Kg·次),正常对照组和肺损伤模型组则在各时间点通过平行注射等量磷酸盐缓冲液。72h后处死各组小鼠,先行支气管肺泡灌洗,并检测支气管肺泡灌洗液(BALF)蛋白含量;留取肺组织标本,行肺组织湿干重比值(W/D)测定和肺组织病理学观察。结果:肺损伤模型组BALF中蛋白含量(0.25±0.09mg/ml)和肺组织W/D(6.13±0.63)均显著高于正常对照组(分别为0.09±0.04mg/ml和4.72±0.63,P<0.01);地塞米松治疗组和APS治疗组BALF蛋白含量(0.13±0.05mg/ml,0.17±0.04mg/ml)、肺组织W/D(5.31±0.48,5.43±0.47)均较肺损伤模型组显著降低,差异有统计学意义(P<0.05)。肺组织病理学结果显示,与正常对照组相比,肺损伤模型组肺泡间隔明显增宽,肺组织有明显炎性细胞浸润、小血管充血、出血,部分肺泡内可见水肿;地塞米松治疗组和APS治疗组肺组织的上述炎性损伤较肺损伤模型组显著改善。结论:APS实验性治疗内毒素血症性肺损伤是可行和有效的。

Objective:To investigate the potential effect of astragalus polysaccharide(APS)on lipopolisaccharide(LPS)-induced endotoxemic lung injury.Method:40 C57BL/6J mice were randomly divided into fours groups:normal control group,lung injury model group,dexamethasone treatment group and APS treatment group,10mice in each group.All mice except those in normal control group were injected intraperitoneally with 5mg/kg LPS to induce endotoxemia.The same volume of phosphate buffered saline(PBS)was injected into mice of normal control Group.2h,24hand 48hafter LPS injection,dexamethasone at dose of 5mg/kg or APS at dose of 200mg/kg was injected intraperitoneally into mice of dexamethasone treatment group and APS treatment group.In parallel,the same volume of PBS was injected intraperitoneally into mice of normal control group and lung injury model group.At the end of 72h,all mice were sacrificed.Bronchoalveolar lavage was operated,bronchoalveolar lavage fluid(BALF)was collected and the protein content was detected.Lung tissues were taken,ratio of lung wet to dry weight(W/D)were detected and pathological changes of lung tissue were observed.Results:Compared with normal control group,protein content in BALF and W/Dwere significantly higher in lung injury model group(0.25±0.09mg/ml vs 0.09±0.04mg/ml,6.13±0.63vs 4.72±0.63,P<0.01).Protein content in BALF and W/D were significantly decreased in dexamethasone treatment group(eg.0.13±0.05mg/ml and 5.31±0.48)and APS treatment group(eg.0.17±0.04mg/ml and 5.43±0.47)compared with lung injury model group(P<0.05).Results of pathological changes in lung tissue have shown that,compared with normal control group,alveolar septum was wider with evident inflammatory cells infiltration,congestion of venule and rhemorrage in lung injury model group.These changes were evidently ameliorated in both dexamethasone treatment group and APS treatment group.Conclusion:Application of APS in endotoxemic lung injury was feasible and effective in experimental study.