低剂量还原型谷胱甘肽可促进人脐血干细胞增殖

Low-dose reduced glutathione promotes umbilical cord blood mesenchymal stem cell proliferation

背景:获得足够数量的细胞是细胞移植治疗中面临的一个关键问题,有研究表明可以通过合理的刺激促进干细胞增殖。目的:观察还原型谷胱甘肽对人脐血间充质干细胞的生物学特性的影响。方法:实验分为两组,对照组为正常人脐血间充质干细胞,实验组为使用0.15 g/L质量浓度的还原型谷胱甘肽处理的人脐血间充质干细胞。结果与结论:MTT实验结果显示,干预后第5,7,9天,实验组细胞的吸光度值明显高于对照组(P<0.05)。流式细胞术显示,实验组还原型谷胱甘肽对人脐血间充质干细胞的表面标志分子CD29\CD44\CD45\CD105的表达无影响。实时PCR结果显示,还原型谷胱甘肽可以促进人脐血间充质干细胞中细胞外信号调节激酶mRNA的表达(P<0.05)。结果证实,质量浓度0.15 g/L的还原型谷胱甘肽可促进人脐血干细胞增殖。

BACKGROUND：How to obtain a sufficient number of cells is one of the key issues in the celltransplantation therapy, and studies have shown that stem cellproliferation can be promoted by reasonable stimulus. OBJECTIVE：To investigate reduced glutathione effects on biological characteristics of human umbilical cord blood mesenchymal stem cells. METHODS：The cells were divided into two groups：the control group consisted of the normal human umbilical cord blood mesenchymal stem cells, and in the experimental group, human umbilical cord blood mesenchymal stem cells were treated with 0.15 g/L reduced glutathione. RESULTS AND CONCLUSION：At days 5, 7, 9, cells treated with 0.15 g/L reduced glutathione showed higher absorbance values than those in the control group （P＆lt;0.05）. Flow cytometry showed reduced glutathione had no effects on CD29, CD44, CD45, CD105 expression. Real-time PCR results showed reduced glutathione was capable of promoting extracellular signal-regulated kinase mRNA expression （P＆lt;0.05）. Findings from this study showed that 0.15 g/L reduced glutathione can promote the proliferation of human umbilical cord blood mesenchymal stem cells.