季节性流感疫苗免疫血清与H7N9禽流感病毒交叉反应

Analysis of cross-protective immunity of neutralizing antibodies to H7N9 influenza virus raised in sera vaccinated with seasonal influenza vaccine

目的分析接种季节性流感疫苗人群是否具有对新发H7N9流感病毒具有交叉保护,并反向检测了雪貂产生H7N9抗血清与H1/H3/H5不同亚型流感病毒的免疫反应。方法利用血凝抑制试验和微量中和实验检测疫苗免疫后人群转阳血清对A/Anhui/1(H7N9)病毒中和作用及雪貂H7N9抗血清与A/California/07/2009(H1N1)、A/PR/8/34(H1N1)、A/Brisbane/59/2007(H1N1)、A/Brisbane/10/2007(H3N2)、A/Shenzhen/406H/2006(H5N1)和A/Vietnam/1203/2004(H5N1)等不同亚型病毒的中和作用。结果季节性流感疫苗受众者转阳血清与H7N9病毒反应HI及中和抗体反应均为阴性,雪貂H7N9抗血清与H1/H3/H5不同亚型流感病毒反应HI及中和抗体反应均为阴性。结论季节性流感疫苗接种人群不具有对新发H7N9流感病毒交叉保护,并且反向验证了H7N9病毒抗血清与H1/H3/H5亚型流感病毒亦无交叉免疫反应。

Objective To investigate whether seasonal vaccine induces anti-virus activity against H7N9 and cross- reactive immune response against related H1/H3/HSsubtype influenza viruses. Methods A/Anhui/1 （H7N9） were pre- incubated with sera vaccinated with seasonal vaccine and A/Califoruia/07/2009 （H1N1）, A/PR/8/34 （H1N1）, A/ Brisbane/59/2007 （ H1 N1 ）, A/Brisbane/10/2007 （H3N2） , A/Shenzhen/406H/2006 （ HSN1 ）, A/Vietnam/1203/2004 （HSNI） were pre incubated with ferret sera infect with HTN9 through Micro-neutralization and HI Assay. Results Human sera from the donors were tested for HI and neutralization activity against influenza （H7N9） preparationsremained at undetectahle levels after seasonal vaccination, and ferret sera were tested for HI and neutralization activity against H1/H3/H5 type influenza viruses either. Conclusion The neutralizing antibodies raised in all donors sera vaccinated with seasonal vaccine cannot neutralize H7N9 influenza virus, and cannot cross-react to H1/H3/H5 subtype.