肝细胞癌中HIF-1α与Glut1表达的关系及意义

Study on expression of HIF-1α and Glut1 in tissues of hepatocellular carcinoma and its significance

目的探讨缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)与葡萄糖转运蛋白1(glucose transporter 1,Glut1)在肝癌组织中的表达关系及其意义。方法应用RT-PCR对28例新鲜肝癌标本和相应癌旁正常肝组织中HIF-1α与Glut1的mRNA表达进行检测。应用免疫组织化学Envision法对47例肝癌和10例相应癌旁正常肝组织中的HIF-1α与Glut1蛋白表达进行检测。结果 RT-PCR和免疫组织化学结果一致。HIF-1α和Glut1在肝癌组织中的阳性率明显高于癌旁正常肝组织(P<0.05)。在肝癌组织中HIF-1α与Glut1的表达均与肿瘤大小、病理分级、临床分期、有无淋巴结转移有关(P<0.05)。肝癌组织中HIF-1α表达越强,Glut1表达也越强,两者的表达呈正相关(r=0.6228,P=0.0004)。结论 HIF-1α和Glut1在肝癌组织中的过表达与肝癌的恶性演进、侵袭和转移有密切关系,两者联合检测有助于判断肝癌分期及评估预后。HIF-1α可能通过上调Glut1的表达促进肝细胞癌的发生。

Objective To investigate the relationship between the expression of hypoxia inducible factor-1α（HIF- 1α） and glucose transporter 1 （Glutl） in hepatocellular carcinoma （HCC） and its clinical significance. Methods RT- PCR was used to detect the expression of HIF-1α mRNA and Glutl mRNA in samples from 28 HCC cases and corresponding tumor-adjacent normal tissues. Immunohistochemical staining Envision method was used to detect the expression of HIF-1α and Glutl in HCC tissues from 47 cases and tumor-adjacent normal tissues from 10 cases. Results The results of RT-PCR were consistent with those of immunohistochemical analysis. The positive expression rates of HIF-1 α and Glutl in HCC tissues were higher than those in tumor-adjacent normal tissues （ P 〈 0. 05 ）. There was association between the expression of HIF-1α and Glutl and tumor size, pathology grade, clinical stage, lymphatic metastasis （P 〈 0. 05）. With the increase of HIF-1α expression, the expression of Glutl increased;positive correlation was found between HIF-1α and Glutl expression（r = 0. 6228,P = 0. 0004）. Conclusions The overexpression of HIF-1α and Glutl in HCC tissues is closely related to the malignant development, invasion and metastasis of HCC. Detection of both HIF-1α and Glutl can facilitate the determination of HCC clinical stage and the evaluation of its prognosis. There may be a mechanism that the expression of Glutl is up-regulated by HIF-1α,which facilitates the origin of HCC.