正畸牙龈炎患者中牙龈卟啉单胞菌致病岛Rag基因的检测及其致病性分析

Detection and pathogenic analysis of rag genotypes of Porphyromonas gingivalis in orthodontic patients with gingivitis

目的:分析不同rag基因型牙龈卟啉单胞菌(Pg)在正畸牙龈炎患者中的分布状况。方法:41例患者为戴用矫治器后发生牙龈炎者(正畸治疗组),36例为未戴矫治器牙周健康者(对照组),25例为中、重度牙周炎患者(牙周炎组)。利用多重PCR技术检测受试者龈沟液标本中牙龈卟啉单胞菌及致病岛rag基因,分析其与临床指标之间的相互关系。结果:牙龈卟啉单胞菌阳性率(%)在正畸治疗组、对照组和牙周炎组分别为70.7、36.1和92.0(P<0.01)。在Pg阳性患者(正畸治疗组和牙周炎组)rag基因型检出率依次为rag-3、rag-4、rag-1和rag-2。结论:不同rag基因型P.gingivalis在不同病变组织中的分布不同;rag-3型和rag-4型Pg与牙龈炎的发生发展有密切关系。

Objective： To investigate the distribution of rag genotypes of Porphyromonas gingivalis （Pg） in orthodontic patients with gingivitis. Methods： Multiple polymerase chain reaction（ Multiple PCR） was set up to detect Pg and rag genotypes of Pg in 41 ortho- dontic patients with gingivitis, 36 controls without periodontal disease and 25 patients with periodontitis. The pathogenicity island rag gene and clinical indicators was analyzed. Results： Pg was positive in 70.7%, 36.1% and 92.0% of the orthodontic patients, healthy controls and patients with periodotitis respectively（P 〈0.01 ）. In the patients with gingivitis or periodontitis the positive Pg rag-3 preva- lence was the highest followed by rag-4, rag-1 and rag-2. Conclusion： Pg with rag-3 and rag-4 may be associated with the develop- ment of gingivitis in the patients with orthodorLtics treatment.