线粒体基因7.4kbp大片段缺失突变与侵袭性牙周炎的关系研究

Correlation analysis between mitochondria DNA 7. 4 kbp large scale deletion and aggressive periodontitis

目的:探讨线粒体基因(mtDNA)7.4 kbp大片段缺失突变和侵袭性牙周炎(AgP)的关系。方法:选取20例AgP患者(AgP组)和20例牙周健康者(对照组),收集外周血及牙周翻瓣术及牙冠延长术中切取的牙龈组织,同时收集10例慢性牙周炎者(CP组)牙周翻瓣术中切取的组织。采用长距离PCR方法检测血样本和牙龈组织样本中mtDNA 7.4 kbp大片段缺失,比较3组间的差异。结果:AgP组20例牙龈组织中1例存在mtDNA 7.4 kbp大片段缺失,AgP组2例组织和CP组1例组织中检测到目前尚未报告过的mtDNA 5 537 bp大片段缺失突变。所有血样和对照者牙龈组织中均未检测到大片段缺失突变。结论:AgP患者牙龈组织中存在mtDNA 7.4 kbp大片段缺失,发现一种新的mtDNA 5 537 bp大片段缺失突变。

Objective： To study the mitochondria DNA（mtDNA） 7.4 kbp deletion in patients with aggressive periodontitis（AgP）. Methods： Blood and gingival tissue samples were collected from 20 patients with AgP（ AgP group）, 20 healthy control subjects （ Control group） and gingival tissue from 10 patients with chronic periodontitis（ CP group）. Long range PCR was used to detect mtD- NA 7.4 kbp deletion in all subjects. Results： MtDNA 7.4 kbp deletion was found in gingival tissue of 1 patient with AgP, a novel mtDNA 5537bp deletion was detected in 2 cases of AgP group and 1 of CP group. No deletion was found in the blood samples of all subjects and in gingival tissue in control subjects. Conclusion： MtDNA 7.4 kbp deletion and a novel mtDNA 5 537 bp deletion in gingival tissue may be related with aggressive periodontitis.