富血小板血浆联合骨髓间充质干细胞对大鼠脊髓损伤后NGF、BDNF的影响

Effect of platelet rich plasma combined with bone marrow mesenchymal stem cells on the expression of nerve growth factor and brain-derived neurotrophic factor in rats with spinal cord injuries

目的观察富血小板血浆(PRP)与骨髓间充质干细胞(BMSCs)联合应用对大鼠脊髓损伤后神经营养因子的神经生长因子(NGF)、脑源性神经营养因子(BDNF)的影响。方法 SD大鼠40只,建立大鼠脊髓损伤模型,术后PRP组在损伤段脊髓注入RPR,BMSCs组注入BMSCs,PRP+BMSCs联合组注入BMSCs+PRP复合物,对照组不注入任何物质。术前3d和术后8周对大鼠后肢的恢复能力进行BBB运动功能评分。造模后第8周,处死动物,HE染色进行病理学检查。蛋白质印迹法(Western blot)检测脊髓中NGF、BDNF蛋白水平的变化。结果与对照组相比,各治疗组大鼠后肢功能恢复具有明显提高,PRP+BMSCs组的大鼠后肢功能恢复最好,差异有显著性意义(P<0.05)。与对照组相比,各治疗组脊髓的NGF、BDFN的蛋白表达水平均有所提高,差异有显著性意义(P<0.05)。结论 PRP、BMSC能恢复脊髓损伤大鼠后肢功能恢复,提高脊髓中NGF、BDNF蛋白水平。

Objective To observe the effect of platelet rich plasma （ PRP） combined with bone marrow mesenchymal stem cells （ BMSCs） on the expression of nerve growth factor （ NGF） and brain-derived neurotrophic factor （ BDNF） in rats with spinal cord injuries.Methods The rat model with spinal cord injury was established using 40 SD rats.After the operation, rats in PRP group were injected with PRP into the spinal cord at the injured segments.Rats in BMSCs group were injected with BMSCs, and rats in PRP＋BMSCs combination group were injected with the combination of BMSCs and PRP, while rats in the control group were treated without any injection.At the 3rd day and the 8th week after the operation, the BBB motor function of the hind limbs of rats was evaluated.At the 8th week after model establishment, the rats were executed and the HE staining was performed for pathological examination.The changes of the expression of NGF and BDNF protein were detected using Western blotting.Results Compared with that in the control group, the function of the hind limbs of rats in each treatment group improved significantly, and the biggest improvement was observed in PRP＋BMSCs combination group （P＆lt;0.05）.The expression of NGF and BDNF protein in spinal cord in each treatment group improved significantly comparing with that in the control group （ P＆lt;0.05 ） .Conclusion PRP and BMSCs can restore the function of the hind limbs of rats with spinal cord injuries and improve the expression of NGF and BDNF protein in spinal cord.