MS-222对大鲵麻醉效果的初步观察

Effectiveness of MS-222 as anesthetic agents for Andrias davidianus

观测MS-222对大鲵（ Andrias davidianus）的麻醉效果。12～15℃水温下，使用0.6 g/L MS-222对16尾4.7龄宁陕县东河的大鲵和16尾2.7龄周至县黑河的大鲵进行浸浴麻醉，每间隔5 min，观测个体的逃匿反应、翻正反射及痛感反应，记录个体麻醉时间和恢复时间并进行数据分析。使用0.6 g/L MS-222对大鲵进行浸浴处理，32尾个体均达到深度麻醉，所需时间为11～30 min，苏醒时间为30～180 min；体重与麻醉时间呈显著的正相关关系（ r＝0.320），水温与麻醉时间呈显著的强负相关关系（ r＝-0.698）。试验过程中没有个体死亡。

This study represents the first attempt to evaluate the effectiveness of MS-222 as anesthetic agents for Andrias davidianus.Two groups of A.davidianus, sixteen from Heihe river basin and sixteen from Donghe river basin , were anes-thetized in a 12 ℃ water bath containing MS-222 （ 600 mg/L of water ） . After a one half month period , eight of the A.davidianus were randomly chosen for anesthesia in a 15℃water bath.Escape, right, superficial pain, and deep pain reflexes were monitored at every five-minute intervals after exposure to anesthetics .The duration of deep anesthesia and of recovery was recorded .MS-222 administered at 0.6 g/L produced deep anesthesia in all of the A.davidianus either in 12 ℃water bath or in 15 ℃ water bath.The time for anesthesia and recovery ranged between 11 ~30 min and 30 ~180 min, respectively .The higher water temperature significantly （ P＆lt;0.01 ） reduced the time required for deep anesthe-sia and for recovery at both groups .A slightly positive correlation （ r=0.320 ） was found between body mass and the time for anesthesia and recovery .