母婴分离/社会击败应激抑郁症小鼠行为学与中枢神经递质的变化及温阳解郁复方的干预作用

Changes of Behaviors and Central Neurotransmitters and Effect of Warming Yang and Soothing Liver Formula on Depression Model Mice Established by Maternal Separation and Social Defeat

目的:建立与验证母婴分离/社会击败应激抑郁症小鼠模型,并研究温阳解郁不同组方对抑郁症的干预效果。方法:用母婴分离与社会击败应激相结合的方法建立小鼠抑郁症模型,并用温阳解郁不同组方进行干预治疗。通过行为学实验(强迫游泳、鼠尾悬挂实验)验证模型,高效液相色谱法检测不同脑区单胺类神经递质的含量。结果:行为学实验结果表明,母婴分离/社会击败小鼠在强迫游泳与鼠尾悬挂实验中不动时间均明显延长。模型小鼠腹侧被盖核(VTA)区去甲肾上腺素(NE)水平明显上升,但未达到显著水平。温阳解郁不同组方均有不同程度的调整作用。结论:从行为学实验的结果看,母婴分离/社会击败模型存在着焦虑、抑郁状态,符合抑郁症模型标准。该模型并不存在神经递质含量的变化,这可能与模型本身的特点有关。

Objective：To establish and verify a kind of depression model and study the effect of Warming Yang and Soothing Liver formula on it. Methods ： The depression model mice were established by maternal separation and social defeat, and treated with Warming Yang and Soothing Liver formula. Behavioral tests were used to verify this model, and high efficiency liquid chroma- tography was used to detect the contents of central neurotransmitters. Results ： The behavioral tests showed that the immobile time of model mice in both forced swimming and tail suspending tests lengthened obviously. The NE levels of model mice were in- creased in ventral tegmental nucleus （P 〉 0. 05 ）, And Warming Yang and Soothing Liver formula had regnlation effects at different degrees. Conclusion：According to the behavioral results, the depression model established by maternal separation and social defeat was in anxious and depressive state,which was consistent with the standard of depression. But the central neurotransmitters were little changed in this kind of model, which may be because of its only characters.