HPLC测定逍遥颗粒中的芍药苷

Determination of Paeoniflorin in Xiaoyao Particles by HPLC

目的：测定不同批次逍遥颗粒中的芍药苷的含量.方法：采用HPLC法,色谱柱为Agilent TC C18（250 mm＆#215;4.6 mm,5μm）;流动相为乙腈-1％磷酸溶液（20∶80）;检测波长为230 nm;柱温为30℃;流速为1.0 ml/min;进样体积10μL.结果：芍药苷1.35～27.00 μg＆#183;mL-1与峰面积呈良好的线性关系（r=0.999）,平均加样回收率为98.55％,RSD=1.8％（n=6）;5批次逍遥颗粒中的芍药苷的含量为0.32％.结论：所用方法简单、稳定,芍药苷的含量可作为逍遥颗粒的品质评价依据之一.

Objective： To determine the content of Paeoniflorin in the Xiaoyao Particles of different batches. Methods： HPLC analysis was adopted with the column ofa Agilent TC C18 （250 mm × 4.6 mm, 5 μm） at 30 ℃ of column temperature. The mobile phase was acetonitrile -1% phosphate （20 ： 80） with detection wavelength of 230 nm and flow rate was 1.0 mLmin-I. The injection volume was 10 μL. Results： Paeoniflorin showed a good linear relationship between area integration value and sampling amount at a range of 1.35-27.00 μg ml-1 （r=0.999）, with the average recovery of 98.55 % and RSD = 1.8 % （n = 6）. The mean content of Paeoniflorin in 5 batches of Xiaoyao Particles was 0.32 %. Conclusion： The method is simple and stable, which can be used as one of the bases of the quality cvaluation of Xiaoyao Particles.