波长切换高效液相色谱法同时测定化胃舒颗粒中4种有效成分的含量

Simultaneous Content Determination of Four Kinds of Effective Constituent in Huaweishu Granules by Wavelength Switching-HPLC

目的 建立同时测定化胃舒颗粒中毛蕊异黄酮苷、阿魏酸、甘草苷和橙皮苷4种成分含量的反相高效液相色谱法.方法 采用Synergi C18柱（250 mm×4.6 mm,4μm）,以乙腈-0.05％磷酸水溶液（体积分数）为流动相进行梯度洗脱,流速为1.0 mL/min,柱温为30℃.采用切换波长的方法,在260 nm下检测毛蕊异黄酮苷,在316 nm下检测阿魏酸,在237 nm下检测甘草苷,在280 nm下检测橙皮苷.结果 毛蕊苷异黄酮苷、甘草苷、阿魏酸和橙皮苷的进样量分别在0.087 5 ～0.437 8 μg（r=1,n=5）,0.085 45 ～0.427 3 μg（r=0.9998,n=5）,0.081 6 ～0.408 μg（r =0.999 9,n=5）和0.123 5 ～0.618μg（r=0.999 8,n=5）范围内与峰面积呈良好的线性关系,平均加样回收率依次为96.84％,98.04％,97.35％和98.10％,RSD分别为0.98％,1.28％,1.31％和1.05％.结论 该方法可同时测定化胃舒颗粒中毛蕊苷异黄酮苷、甘草苷、阿魏酸和橙皮苷的含量.

Objective To develop a RP-HPLC method for the content determination of calycosin-7-glucoside,liquiritin,ferulic acid and hesperidin in Huaweishu Granules.Methods HPLC was performed on the Synergi C18 column（250mm×4.6 mm,4μm） with the gradient elution of acetonitrile-0.05% phosphoric acid aqueous solution at a flow rate of 1.0 mL/min.The column temperature was maintained at 30 ℃.The wavelength switching method was adopted for conducting the detection at 260 nm for calycosin-7-glucoside,at 237 nm for liquiritin,at 316 nm for ferulic acid and at 280 nm for hesperidin.Results The linear ranges were 0.087 5-0.437 8 μg（r=1,n=5）,0.085 45-0.427 3 μg（r=0.999 8,n=5）,0.0816-0.408 μg（r=0.999 9,n=5） and 0.123 5-0.618 μg（r=0.999 8,n=5）,respectively.The recovery rates were 96.84% （RSD=0.98%）,98.04% （RSD=1.28%）,97.35% （RSD=1.31%）and 98.10% （RSD =1.05%）,respectively.Conclusion This method can simultaneously determine the contents of calycosin-7-glucoside,liquiritin,ferulic acid and hesperidin in Huaweishu Granule.