高效液相色谱法测定清宫长春胶囊中芍药苷含量

Determination of Paeoniflorin Content in Qinggongchangchun Capsules by HPLC

目的 探讨清宫长春胶囊质量控制的新方法.方法 采用Kromasil-C18色谱柱（250 mm×4.6mm,5μm）,流动相为甲醇-0.1％磷酸溶液（25∶75）,检测波长为230 nm,流速1.0 mL/min.结果 芍药苷质量浓度在16.08 ～ 241.2 μg/mL范围内与峰面积呈良好线性关系,清宫长春胶囊中芍药苷的平均回收率为98.83％,RSD=1.12％.结论 所用方法简便、快速、准确、灵敏度高、重复性好,可有效控制清宫长春胶囊的质量.

Objective To study the new methods of the quality control of Qinggongchangchun Capsules.Methods The Kromasil-C18 chromatographic column （250 mm ×4.6 mm,5 μm） was adopted.The mobile phase was methanol-0.1% phosphoric acid solution（25 ∶75）,the detection wavelength was 230 nm,the flow rate was 1.0 mL/min.Results The mass concentration of paeoniflorin in the range of 16.08-241.2 μg/mL showed the good linear relation with the peak area.The recovery rate of paeoniflorin was 98.83% with RSD =1.12%.Conclusion The used method is simple,rapid and accurate with high sensitivity and good repeatability and can effectively control the quality of Qinggongchangchun Capsules