IL-8、TNF-α在Hp感染中的作用研究

Study on the effect of IL-8 and TNF-α in Helicobacter pylori infection

目的 研究幽门螺杆菌 (Hp)相关性胃肠疾病时胃粘膜组织IL 8、TNF α的活性水平及其病理作用。方法 采用酶联免疫吸附试验夹心法检测胃粘膜组织中IL 8、TNF α的水平 ,并观察IL 8、TNF α水平与胃粘膜炎症活动的关系。结果 1、Hp感染的胃粘膜组织中 ,IL 8或TNF α的浓度明显高于非Hp感染患者。 2、IL 8、TNF α在Hp相关性胃炎与十二指肠球溃疡患者胃粘膜组织的表达无显著性差异。 3、在Hp感染患者中 ,有炎症活动的患者其胃粘膜组织中IL 8、TNF α的表达明显高于无炎症活动的患者。4、Hp感染患者胃粘膜组织中IL 8的表达与TNF α水平呈正相关。结论 炎性细胞因子IL 8、TNF

Objective To investigate the activity of interleukin-8 (IL-8) and tumour necrosis factor-α (TNF-α) and their pathological mechanism in patients with H.pylori associated gastroduodenal disease.Method The production of IL-8 and TNF-α by humun antral mucosa during short term culture in vitro has been measured by ELISA sandwich,and the relationship between the concentrations of IL-8 and TNF-α and gastric mucosa inflammatory activity were observed.Result 1.The concentrations of IL-8 and TNF-α in culture supernatants were significantly greater in H.pylori-positive patients than in H.pylori-negative patients (P<0.01).2.In H.pylori infected cases,there is no marked difference in the concentration of IL-8 and TNF-α between patients with chronic gastritis and those with duodenal ulcer (P>0.05).3.In cases with H.pylori infection,the expression of IL-8 and TNF-α in patients with active gastritis were significantly higher compared with those with inactive gastritis(P<0.01).4.There was a positive correlation (r=0.5484,P<0.01) between the mucosal expression of IL-8 and the TNF-α in patients with H.pylori infection in culture supernatants.Conclusion These results suggest a possible pathogenetic role for IL-8 and TNF-α in H.pylori-αssociated gastroduodenal disease.