乙型肝炎患者胃粘膜中HBsAg表达分析

Study on the expression of HBsAg in gastric mucosa of patients with hepatitis virus B

目的 探讨乙型肝炎病毒的泛嗜性。方法 采用免疫组化SP法 ,对 45例随机采样的乙型肝炎患者行胃粘膜活检 ,作HBsAg检测 ;采用ELISA法检测静脉血HBV。结果 乙型肝炎患者胃粘膜中有HBsAg存在 ,主要呈弥漫胞浆型分布 ,腺腔和胃小凹及间质中也可见HBsAg颗粒 ;血清中HBV单项或多项阳性者 ,胃粘膜HBsAg阳性率相近 (75～ 80 % ) ;各类型乙型肝炎患者胃粘膜HBsAg阳性率比较 :CAH >CPH >HLC ,但彼此之间差异不显著 (P >0 0 0 5 )。结论 乙肝患者胃粘膜中有HBsAg存在。

Aim To observe if there was HBsAg in the gastric mucosa of patients with hepatitis B. Methods Forty-five patients with hepatitis B were collected by random sampling method.HBsAg was detected using immunocytochemistry method (SP) in the gastric mucosa acquired by gastroscope;HBV was detected using ELISA in sera. Results There was HBsAg in the gastric mucosa of patients with hepatitis B.HBsAg mainly lies in the cytoplasm,and also in gastric pit,peptic glandular ducts and gastric extracelluar matrix.Of the one or more positive markers of HBV in sera,the postive rates of HBsAg in the gastric mucosa were similar(75～80%).The order of the postive rates of HBsAg in the gastric mucosa was CAH>CPH>HLC,but the differences were not statistically significant (P>0.05). Conclusion There were HBV in the gastric mucosa of patients with hepatitis B.