西沙比利对大鼠胃粘膜血流影响的实验研究

Effect of Cisapride on blood flow of gastric mucosa in rats

目的 本文研究了西沙比利对大鼠胃粘膜血流的影响 ,并进而探讨其可能的作用机制。方法 3 2只Wistar大鼠随机分为对照组 ,西沙比利 0 5mg/kg组 ,1mg/kg组和 2mg/kg组 ,采用中性红分泌法和grees -reactin方法检测胃粘膜血流及一氧化氮含量。结果 西沙比利 1mg/kg组大鼠胃粘膜血流量为 (0 7± 0 13mL/min) ,显著高于空白对照组 (0 45± 0 18mL/min)和西沙比利0 5mg/kg组 (0 4± 0 2 4mL/min) (P <0 5 0 ) ,且西沙比利 1mg/kg组胃粘膜一氧化氮含量亦显著高于空白对照组 (2 3 3 2± 7 40 μmol/L/mgvs 16 93± 3 87μmol/l/mg)和西沙比利 0 5mg/kg组 (16 76± 1 0 6μmol/L/mg) (P <0 0 5 )。西沙比利 2mg/kg组胃粘膜血流量为 (0 3± 0 17mL/min) ,显著低于空白对照组 (0 45± 0 .18mL/min) (P <0 0 5 ) ,其一氧化氮含量 (4 3 5± 1.5 2 μmol/L/mg)亦显著低于空白对照组 (P <0 0 5 )。结论 西沙比利通过影响胃粘膜内一氧化氮含量从而改变胃粘膜血流 ,西沙比利 1mg/kg对大鼠胃粘膜具有保护作用。

Aim To study the effect of cisapride on rat gastric mucosal blood flow and its mechanism. Methods Thirtytwo rats were devided into control group,cisapride 0.5mg/kg group,1mg/kg group and 2mg/kg group.After the gastric chamber was prepared,we examed the gastric mucosal blood flow with the neutral red secretory method,furtherly examed the volume of nitric oxide with the grees-reaction method. Results The gastric mucosal blood flow in cisapride 1mg/kg group (0.7±0.13mL/min) was significantly higher than that in control group (0.45±0.18mL/min)and cisapride 0.5mg/kg group(0.4±0.24mL/min)(P<0.05).The volume of nitric oxide in cisapride 1mg/kg group was significantly higher than that in control group (23.32±7.40μmol/L/mg vs 16.93±3.87μmol/L/mg)and cisapride 0.5mg/kg(16.76±1.06μmol/L/kg)(P<0.05).The gastric mucosal blood flow in cisapride 2mg/kg group was significantly lower than that in control group(0.3±0.17mL/min vs 0.45±0.18mL/min)(P<0.05),so did the nitric oxide(4.35±1.52μmol/L/mg vs 16.93±3.87umol/L/mg)(P<0.05).Conclusion Cisapride has the effect on gastric mucosal blood flow.This effect is associated with the secretory of nitric oxide.Cisapride 1mg/kg can protect the gastric mucosa properly.