MTT法检测非小细胞肺癌对紫杉醇的敏感性

MTT assay for detecting paclitaxel chemosensitivity on non small cell lung cancer

目的探讨四氮唑蓝比色分析法(MTT法)用于指导临床个体化化疗药物选择的实用性,为非小细胞肺癌临床化疗用药提供参考。方法培养肺癌A549细胞,加入不同浓度的紫杉醇,作用24、48、72、96 h,用MTT法计算细胞生长抑制率,测定其对肿瘤细胞的体外敏感性。结果不同浓度的紫杉醇对A549细胞的生长均有一定的抑制作用,且呈剂量依赖性和时间依赖性。结论 MTT法检测有助于指导临床选择化疗药物剂量,提高化疗效果,减少毒副作用,为非小细胞肺癌临床个体化化疗方案的选择提供客观依据。

Objective To assess the feasibility of MTT colorimetric assay for testing the in vitro chemosensitivity of non small cell lung cancer cells to paclitaxel（ PTX）. Methods A549 cells were exposed to paclitaxel at different concentrations or different treatment times, and then the growth - inhibiting effects were determined by MTI＂ assay. Results Paclitaxel in- hibited the growth of A549 cells significantly in time - and dose - dependent manners. Conclusion MTT assay may help select appropriate chemotherapeutic agents and provides evidence for individualized chemotherapy for non small cell lung cancer.