血管性痴呆载脂蛋白E基因多态性分析

An analysis of apolipoprotein E polymorphism in patients with vascular dementia

目的 探讨载脂蛋白E基因多态性与血管性痴呆的相互关系并对血管性痴呆 (VaD)与老年性痴呆 (AD)基因型进行比较。方法 采用限制性片段长度多态性分析———多聚酶链式反应法 (RFLP PCR)检测了一组VaD患者ApoE基因多态性并与一组AD患者及一组同龄健康人对照组作了比较。结果 VaD患者中 3/ 4型频度和ε4等位基因频度均明显低于AD患者组 (0 .118比 0 .319,P <0 .0 5 ;0 .0 5 89比 0 .180 9,P <0 .0 1) ,VaD组的 3/ 4型及ε4频度与同龄健康人对照组相比差异无显著性意义。年龄亚组分析显示 :VaD组和AD组在 3/ 4型和ε4频度上的差异主要表现在 6 0～ 6 9岁年龄组 (P <0 .0 0 1)。结论 VaD组ε4频度与健康对照差异无显著性意义 ,而明显低于AD组 ,ε4可能并未参与VaD致病过程 ,而VaD与AD可能存在不同的遗传学发病机制。

Objective To study the relation between ApoE alleles and the occurrence of VaD and to compare the alleles in VaD and Alzheimer's disease(AD).Methods ApoE genotypes were analysied by using polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) technique in DNA separated from 51 patients with VaD and 47 patients with AD.The relative frequency of the ε4 alleles and genotypes were estimated in these two patient groups and their data were compared with those from a group of age and sex matched healthy subjects.Results The frequencies of the ApoE ε4 and 3/4 genotype in VaD group were lower than those in the AD group(0.0589 vs 0.1809,P=0.013;0.118 vs 0.319,P=0.025).No significant differences were found when data of VaD were compared with those in the healthy subjects.In AD group,the genotypes of 3/4 and ε4 alleles were predominant in 60～69 years age subgroup.Conclusions Apo Eε4 allele is a genetic risk factor for AD,while it may be not for VaD.The relationship between ε4 and VaD should be further studied.