15种竹叶中碳苷黄酮的含量研究

Research on contents of flavone C-glycosides in the fifteen cultivars bamboo leaves

为找到富含竹叶黄酮的竹类资源.采用高效液相色谱-紫外分光光度法（HPLC-UV）同时测定刚竹属15种竹子中6种碳苷黄酮的含量,Eclipse XDB-C18色谱柱（4.6 mm×250 mm,5μm）,流动相混合溶剂A（乙腈）和溶剂B（体积分数0.2％乙酸水）梯度洗脱条件：10％ A（34 min）,10％～13％ A（34～52 min）,13％～15％ A（52～85 min）,15％～10％ A（85～90 min）,流速1.0 mL/min,检测波长330 nm,柱温30℃,进样量20 μL.结果表明：5种竹子：毛竹、淡竹、天目早竹、龟甲竹和斑竹富含碳苷黄酮类化合物,可作为碳苷黄酮的主要来源.

To find more and new species of bamboo which are rich in bamboo leaf flavones,a strategy for analyzing flavone C-glycosides in the leaves of different cultivars of bamboo was developed.An HPLC-UV method was optimized and validated for determination of six flavone Cglycosides in bamboo leaves from 15 cultivars of Phyllostachys.Chromatographic separations of these compounds were performed on an Ecilipse XDB-C18 column （5 μm,250 mm×4.6 mm i.d.）.A gradient programme was used with the mobile phase,combining solvent A （acetonitrile） and solvent B （0.2％,acetic acid adjusted to pH 3.0） as follows：10％ A（34 min）,10％～13％ A （34～52 min）,13％～15％ A（52～85 min）,15％～10％ A（85～90 min）.The flow rate was 1.0 mL/min,the injection volume was 20 μL and the column temperature was maintained at 30 C.Signal was monitored at 330 nm with UV.Five cultivars （P.heterocycla,P.glauca,P.tianmuensis,P.pubescens and P.bambusoides） are rich in flavone C-glycosides.