抗氧化乌拉尔甘草内生菌株的筛选及鉴定

Screening and identification of antioxidative endophytic strains in Glycyrrhiza uralensis

分离乌拉尔甘草内生真菌,研究乌拉尔甘草内生真菌的抗氧化活性,为内生真菌天然产物的进一步研究提供理论依据.利用组织分离法从乌拉尔甘草根、茎、叶中共分离获得14株内生真菌,其中菌株L1-1,L2-1,R2-1,S2-1对DPPH自由基的清除率达95%以上,铁离子还原法检测菌株L1-2,S2-2,R2-3具有较高还原力,FRAP值分别为256,309,298.通过综合比较,菌株R2-3表现出较强的抗氧化活性,测定活性菌株26SrDNA序列及构建其系统发育树,所筛选的活性菌株R2-3被鉴定为炭疽菌.

Endophytic strains were isolated from roots,leaves and stems of Glycyrrhiza uralensis,antioxidation of endophytic strains were investigated,the results might provide a theoratical basis for the natural products research of endophytic fungi from Glycyrrhiza uralensis.Fourteen endophytic strains were isolated from Glycyrrhiza uralensis,the endophytic strains L1-1,L2-1,R2-1,S2-1 exerted the high antioxidant activity by DPPH,the endophytic strains L1-2,S2-2,R2-3 exerted the high reduction by FRAP.The strain R2-3 was identified as a Colletotrichum gloeosporioides based on colony characteristics and morphological characters by microscopy and 26S rDNA sequence.