微生物酶催化碳氢键的不对称氧化反应合成手性4-氯二苯基甲醇(英文)

Asymmetric synthesis of chiral 4- chlorobenzhydrol by C- H bonds oxidation with microbial enzymes

目的使用具有甲苯降解能力的假单孢菌株作为生物催化剂,通过不对称氧化反应实现手性4-氯二苯甲醇的对应选择性合成。方法采用悬浮细胞生物转化法用于4-氯二苯甲烷的不对称氧化反应研究,产物通过手性液相色谱进行分析。结果得到了5.5%的产率和具有89%的对映选择性的手性4-氯二苯基甲醇。结论通过生物催化实现了4-氯二苯甲烷苄位C-H键的不对称羟基化反应,合成了手性的4-氯二苯基甲醇。

Objective Toluene -degrading Pseudomonas monteilii TA -5 was employed as a biocatalyst for syn- thesis of chiral 4 - chlorobenzhydrol （CPML） with high enantioselectivity by asymmetric oxidation. Methods The suspending whole -cells biotransformation was used for asymmetric oxidation of 4 -chlorodipheylmethane （ CPM）, and the bioproduct were analyzed by chiral HPLC. Results chiral 4 - chlorobenzhydrol （CPML） was obtained with 5.5% yield and 89% ee（ enatiomeric excess）. Conclusion The biocatalytic oxidation can be used for the benzylic C - H bond hydroxylation of CPM to synthesize chiral CPML.