活检印片应用AgNORs技术减少鼻咽癌误诊的意义

The Significance on Reducing Misdiagnosis of Nasopharyngeal Carcinoma Using AgNORs Method in Printed Slides of Biopsy Tissue

目的 :收集 1998- 0 6 / 1999- 10本院 10 0例鼻咽癌活检组织印片 ,探讨提高鼻咽癌细胞学诊断方法。方法 :应用鼻咽癌活检组织印片及 Ag NORs染色技术 ,显微镜观察结果。结果 :男 80例 ,女 2 0例 ,男∶女 =4∶ 1,男最大 70岁 ,最小 2 3岁 ,高峰年龄 30岁～ 5 0岁。患者 93%鼻咽有病灶 ,80 %颈淋巴结肿大。活检印片 HE常规制片 ,可疑者加 Ag NORs胶银染色可鉴别诊断。检查结果 97%找到癌细胞。 3例阴性者核对 HE切片亦为阴性。HE切片检查 91%确诊为鼻咽低分化鳞癌 ,1例诊为恶性淋巴瘤 ;6例为慢性鼻咽炎 ,1例报告坏死物及血性炎症分泌物。另 1例报告坏死物及肉芽组织增生。结论 :文中提出印片技术优点是经济省时 ,设备简单 ,便于基层医院推广应用 ,可减少鼻咽癌患者误诊率。

Objective:To collect 100 cases of nasopharyngeal carcinoma(NPC)from June of 1998 to October of 1999.The aim is to seek a method promoting cytological of NPC.Methods:The printed slides of NPC tissue was stained using AgNORs method and then watched under microscope.Results:Selected patients include 80 cases of the males and 20 cases of the females.The age of patients ranged from 23 to 70 years old.93% patients have nasopharyngeal lesion.The 80% have enlarged lymph nodes of neck.Carcinoma were found in 97% printed slides.3 cases of printed slide for negation of NPC correspond to negative tissue slides.Using HE stain,91% cases were definitely diagnosed as NPC.1 case as lymphoma,6 cases chronic nasopharyngitis,1 case as necrotic and blood ingredient.Another case was reported as necrosis and granuloma.Conclusion:The advantage of printed slides are econmizing,serving time,just needing simpleapprartus,convenient to popularize in basc hospital.In addition,it can lower the misdiagnosis rate of NPC.