摘要
目的探讨连接蛋白43(Cx43)在局灶性脑皮层发育不良(focal cortical dysplasia,FCD)致痫机制中的作用。方法选择手术治疗的颞叶FCD 43例作为观察组,同期正常人脑标本10例作为对照组,采用免疫组织化学方法比较两组Cx43表达情况。结果免疫组织化学检测结果显示观察组平均每个视野Cx43免疫反应阳性细胞为(19.5±3.3)个,对照组平均每个视野Cx43免疫反应阳性细胞为(7.6±3.7)个,观察组明显高于对照组,差异有统计学意义(P<0.05);单纯型FCD患者平均每个视野Cx43免疫反应阳性细胞为(14.5±4.3)个,结合型FCD患者平均每个视野Cx43免疫反应阳性细胞为(22.6±3.8)个,结合型FCD患者Cx43表达较单纯型FCD患者明显增高,差异有统计学意义(P<0.05)。结论 Cx43参与FCD致痫机制,结合型FCD和单纯型FCD致痫机制可能存在差异。
Objective To explore the effect of connexin 43 （Cx43） on epileptogenic mechanisms in patients with fo- cal cortical dysplasia （FCD）. Methods A total of 43 cases （observation group） of FCD in temporal lobe underwent surgical treatment, and 10 normal cerebric samples （control group） at the same period were selected, and eonnexin 43 expression in the two groups was compared with immunohistochemical method. Results Immunohistochemistry detection showed that the average positive Cx43 immunoreactive cells of each visual field was 19.5± 3.3 in observation group and 7.6 ± 3.7 in control group respectively, and the number in observation group was significantly higher than that in control group （ P 〈 0.05 ） ; the average positive Cx43 immunoreactive cells of each visual field in patients with simple FCD was 14.5 ± 4.3 and 22.6 ± 3.8 in patients with combined FCD respectively, and the number in patients with simple FCD was significantly higher than that in pa- tients with combined FCD （P 〈 0.05）. Conclusion Cx43 may be involved in the epileptogenic mechanisms of FCD, and ep- ileptogenic mechanism of simple FCD may be different from that of combined FCD.
出处
《临床误诊误治》
2014年第2期85-88,共4页
Clinical Misdiagnosis & Mistherapy
基金
河北省2013年医学科学研究重点课题计划项目(20130681)
