海蜇生殖腺酶解物抗氧化活性的研究

Antioxidant Activity of Enzymatic Gonad Hydrolysates from Jellyfish Rhopilema esculentum

为提高海蜇的综合利用率,利用木瓜蛋白酶酶解技术制备海蜇生殖腺酶解物。分析酶解物的氨基酸组成,并通过体外化学模拟体系评价酶解物与Fe2+螯合前后的抗氧化活性。试验结果表明,海蜇生殖腺酶解物含有17种氨基酸,具有一定的Fe2+螯合能力和DPPH·清除能力,其螯合率和清除率达到50%时的质量浓度分别为1.73mg/mL和22.20mg/mL。同时,具有一定的还原能力,其在700nm处吸光值达到0.5时的质量浓度为17.61mg/mL。海蜇生殖腺酶解物与Fe2+螯合后,抗氧化能力显著增强,DPPH·清除率达到50%和在700nm处吸光值达到0.5时的质量浓度分别为0.15mg/mL和0.86mg/mL。

The enzymatic hydrolysates of gonad in jellyfish Rhopilema esculentum were prepared by papain, and the amino acid composition and the antioxidant activities of the hydrolysates were analyzed by Fe2＋-chelating assay, 1,1-diphenyl- 2-picrylhydrazyl （DPPH · ） assay and reducing power assay to make full use of the jellyfish. The results showed that the hydrolysates from jellyfish gonad were composed of 17 amino acids, and possessed Fe2＋ -chelatin ability, DPPH radical scavenging capacity with IC10 values of 1. 73 and 22. 20 mg/mL, respectively, and reducing power with AC0.s value of 17. 61 mg/mL. The antioxidant activities of enzymatic hydrolysates, chelated with Fe2＋ , were obviously improved, with the ICs0and ACo.5 values of 0. 15 mg/mL in DPPH radical scavenging capacity and 0.86 mg/mL in reducing power. The findings provide theoretical support for the further exploitation of jellyfish gonad.