细菌脂多糖处理对斑马鱼补体基因表达水平的影响

Effect of Bath of Lipopolysaccharides Derived from Bacteria on Expression of Complement Genes in Zebrafish

将斑马鱼饲养在25～27℃连续充气的自来水中,取体质量约0.2g的健康鱼,用20μg/mL的细菌脂多糖溶液浸泡处理24h,以实时荧光定量PCR技术分析细菌脂多糖处理对斑马鱼体内主要补体基因表达水平的影响。细菌脂多糖处理后,斑马鱼体内的补体成分1r/s、成分4、成分6和甘露聚糖结合凝集素的表达水平逐步升高,且均在12h达到最高值,之后开始下降。但细菌脂多糖处理后,补体成分3和补体因子Bf的表达水平先降低,然后开始升高,表明斑马鱼体内补体系统能够在较短时间内对外界感染作出快速应答,但因补体成分的功能不同,其对外界感染的应答模式也有所不同。

The healthy zebrafish（Danio rerio） with body weight of about 0.2 g were reared in well-aerated tap water at 25-27 ℃, and then bathed in lipopolysaccharide （LPS） solution at a dose of 20 tLg/mL for 24 h. The expression levels of main complement genes in the zebrafish were analyzed by real-time fluorescent quantitative PCR. It was shown that the expression of Clr/s, C4, C6 and MBL was all up-regulated after LPS treatment, with the peak at 12 h and then decline. In contrast, down-regulation of C3 and Bf expression was detected after treatment, and then up-regulation was observed with the peak at 12 h. The findings indicated that the complement system effectively responded to infection in a short time, and various genes respond to the external stimulation in different ways, which might be attributed to the different roles of complement components and complement pathways.