c-myc和c-fos的阳性表达与肝细胞癌发生的相关性研究

Correlation of proto-oncogene c-myc and c-fos expression with the occurrence of hepatocellular carcinoma

目的:探讨c-myc和c-fos的阳性表达与肝细胞癌(HCC)发生的相互关系及临床意义。方法:运用免疫组化检测技术,分别检测肝癌组织、癌旁组织、正常肝脏组织中c-myc和c-fos编码蛋白的表达。结果:正常肝组织c-myc和c-fos均无阳性表达,而肝癌、癌旁组织均出现不同程度c-myc和c-fos表达。c-myc在癌组织表达率为51.43%(36/70),癌旁组织表达率为34.29%(24/70),c-fos在癌组织表达率为20.00%(14/70),癌旁组织表达率为7.14%(5/70),二者比较,差异具有统计学意义(P<0.05)。研究还发现,肝癌组织中c-myc和c-fos的表达存在相关性,一种基因的表达会导致另一种基因表达的升高。c-myc和c-fos癌基因的表达与HCC的发病年龄、性别、肿瘤大小无关,但与HCC的分化程度、UICC分期有关,差异具有统计学意义(P<0.05)。结论:癌基因c-myc、c-fos表达增强与HCC的发生、发展有密切关系。

Objective= To investigate the relationship between the positive expression of proto-on- cogene c-myc and c-los expression in the occurrence of hepatocellular carcinoma （HCC）. Methods- Immu- nohistochemical detection techniques were used to detect expression of c-myc and c-los in hepatocellularcarcinoma tissues, adjacent tissues and normal liver tissues. Results. Positive expression of c-myc and c- los was detected in both carcinoma and adjacent tissues, but not in normal liver tissues. Ratio of c-myc ex- pression in hepatocellular carcinoma and adjacent tissues were 51.43~//00 （36/70） and 34.29~ （24/70）. The proportion of c-los expression in hepatocellular carcinoma tissues and adjacent tissues were 20.00~ （14/ 70） and 7.14~ （5/70）. It showed significant differences between two groups （P^0.05）. The expression of c-myc and c-fos were closely related in the presence of hepatocellular carcinoma. Increased expression of one gene should result in elevated expression of the other. But the expression of c-myc and c-los oncogene was not related with patients age, gender and tumor size, it was correlated with the degree of differentia- tion of HCC and UICC stage （P%0.05）. Conclusion： Increased expression of proto-oncogene c-myc and c- fos is correlated with the occurrence of live cancer.