期刊文献+

双嘧达莫对多发性硬化症的治疗作用及机制的研究 被引量:1

Therapeutic effects of Dipyridamole on MS and its mechanism
下载PDF
导出
摘要 目的:利用双嘧达莫对复发缓解型多动性硬化症(multiple sclerosis,MS)患者的治疗效果和人脑小胶质细胞体外培养,探索小胶质细胞在MS炎症性病理中的作用。方法:将80例患者随机分为两组,治疗组服用双嘧达莫缓释胶囊和强的松,辅助指导性功能锻炼进行治疗;对照组服用安慰剂和强的松,辅助指导性功能锻炼进行治疗。另外建立人脑中小胶质细胞体外培养模型,分为对照组、脂多糖(Lipopolysaccharides,LPS)组和双嘧达莫组,培养24h后收集培养液做ELISA检测多种细胞因子含量。结果:服用双嘧达莫缓释胶囊的治疗组患者总有效率为34%,高于对照组总有效24%,差异具有统计学意义(P<0.05);治疗组在临床症状、神经功能特征和EDSS评分方面的改善均优于对照组,差异具有统计学意义(P<0.05)。细胞培养结果显示LPS处理小胶质细胞导致细胞培养液中白介素-6(IL-6)、白介素-10(IL-10)、白介素-12(IL-12)、白介素-1RA(IL-1RA)、单核细胞趋化因子蛋白-1(MCP-1)和肿瘤坏死因子-α(TNF-α)含量增高,并且这种增高效应能够被双嘧达莫抑制,差异具有统计学意义(P<0.05)。结论:双嘧达莫对MS患者具有治疗效果,并且该疗效是通过抑制中枢神经中的小胶质细胞释放的各种炎性细胞因子产生的,从而证实了小胶质细胞可能参与了MS的发生,并在MS炎症性病理中发挥着积极重要的作用。 Objective: To investigate the therapeutic effect of Dipyridamole on relapsing-remitting hyperactivity sclerosis (MS),and to explore the role of microglia in MS inflammatory pathogenesis by culturing human brain microglia cells in vitro. Methods : A total of 80 cases were randomly divided into treatment group and control group. The treatment group was treated with oral Dipyridamole and Prednisone plus instructional physical exercise, the control group was given placebo, Prednisone and instructional physical exercise. In vitro cultured human brain microglia cells were divided into control group, LPS group and Dipyridamole group. After 24 h of culture, media were collected to do ELISA for multiple cytokines assay. Results: Patients in treatment group showed total effects of 34%,significantly higher than 24% of the control group (P〈C0, 05). The treatment group showed superior results in clinical symptoms, neuro- 454 海南医学院学报Vol. 20 No. 4 Apr. 2014logical features and EDSS scores than the control group (P〈C0. 05). Furthermore,the level of IL-6,IL-10, IL-12,IL-IRA,MCP-1 and TNF-a in culture media treated with LPS were elevated, which can be inhibited by Dipyridamole (P〈C0. 05). Conclusion: Dipyridamole shows therapeutical effects on MS through inhibiting the release of cytokines from microglia cells in CNS. So microglia cells may participant in MS inflammatory pathogenesis and play a crucial role in MS inflammatory pathogenesis.
作者 陈灏 杜小波
出处 《海南医学院学报》 CAS 2014年第4期453-457,共5页 Journal of Hainan Medical University
基金 四川省技术创新资助项目(2011CD00238)~~
关键词 双嘧达莫 多动性硬化症(MS) 小胶质细胞 细胞因子 Dipyridamole; MS; microglia; Cytokine
  • 引文网络
  • 相关文献

参考文献17

  • 1Kurtzke JF. Rating neurologic impairment in multiple sclerosis:an expanded disability status scale (EDSS)[J].{H}NEUROLOGY,1983,(11):1444-1452.
  • 2Noseworthy JH,Lucchinetti C,Rodriguez M. Multiple sclerosis[J].{H}New England Journal of Medicine,2000,(13):938-952.
  • 3McDonald WI,Compston A,Edan G. Recommended diagnostic criteria for multiple sclerosis:guidelines from the International Panel on the diagnosis of multiple sclerosis[J].{H}Annals of Neurology,2001,(1):121-127.
  • 4Steinman L. Multiple sclerosis:a two-stage disease[J].{H}Nature Immunology,2001,(9):762-764.
  • 5Hemmer B,Nessler S,Zhou D. Immunopathogenesis and immunotherapy of multiple sclerosis[J].{H}NEUROLOGY,2006,(4):201-211.
  • 6Compston A,Coles A. Multiple sclerosis[J].{H}LANCET,2008,(9648):1502-1517.
  • 7Scolding N,Franklin R,Stevens S. Oligodendrocyte progenitors are present in the normal adult human CNS and in the lesions of multiple sclerosis[J].{H}BRAIN,1998,(Pt 12):2221-2228.
  • 8Scolding N J,Rayner PJ,Compston DA. Identification of A2B5-positive putative oligodendrocyte progenitor cells and A2B5 positive astrocytes in adult human white matter[J].{H}NEUROSCIENCE,1999,(1):1-4.
  • 9Kerschensteiner M,Bareyre FM,Buddeberg BS. Remodeling of axonal connections contributes to recovery in an animal model of multiple sclerosis[J].{H}Journal of Experimental Medicine,2004,(8):1027-1038.
  • 10Martins TB,Rose JW,Jaskowski TD. Analysis of proinflammatory and anti-inflammatory cytokine serum concentrations in patients with multiple sclerosis by using a multiplexed immunoassay[J].{H}American Journal of Clinical Pathology,2011,(5):696-704.

同被引文献10

  • 1TSUDA M, INOVEK, SALTE M W. Nenropathic pain a- nd spinal microglia a big problem from molecules in "small"glia[ J ]. Trends Neuroci, 2005,28 (2):107.
  • 2ZHANG Z, ZHANG Z Y, FAUSERU, et al. Mechanical a- llodynia and pinal up regulation of P2X4 receptor in experimental auto immune neuritis rats [ J ] Neuroscience, 2008,152(2):495-501.
  • 3TSUDA M,INJOUE K. Neuropathic pain and ATP recep- tors in spinal microglia [J]. Brain Nerve,2007,59 (9): 953-959.
  • 4KIM S H, CHURG M. An experimental model for periphe- ral neuropathy produced by segmental spinal nerve ligationin the rat[J]. Pain, 1992,50:355-363.
  • 5CHAPLAN S R,BACH F W,POGREL J W,et al. Quantitive assessment of tactile allodynia in the rat paw [J]. J Neurosie Methods, 1994,53( 1 ):55-63.
  • 6SWEITZE S M, SCHUBERT P, DELEO J A. Propentofyli- ne,a glial modnlating agent,exhibits antiallodynic properties in rat model of neuropathic pain [J]. J Pharmacol Exp Ther, 2009,297 ( 3 ) : 1 210- 1 217.
  • 7INOUE K, TSUDA M, KOIZUMI S. ATP-and adenosine- ediated-signaling in the central nervous system:chronic pain and microglia: involvement of the ATP receptor P2X4 [ J ]. J Pharmacol Sci, 2004,94:112- 114.
  • 8MULLER C E. Emerging structures and ligands for P2X3 and P2X4reeeptors-towards novel treatments of neuropathic pain [J]. Purinergic Signal,2010,6(2):259-269.
  • 9彭伟,张咸伟,田学愎,邓乾,姚文龙,曹菲,田玉科.永生化小鼠小胶质细胞P_2X_4siRNA靶点的筛选[J].华中科技大学学报(医学版),2008,37(5):657-660. 被引量:4
  • 10于熙,田国刚,田毅.乌司他丁的脑保护作用研究进展[J].海南医学院学报,2010,16(7):948-950. 被引量:6

引证文献1

二级引证文献1

;
使用帮助 返回顶部