肾移植受者并发重症巨细胞病毒感染时T淋巴细胞亚群动态变化

The dynamic changes in T lymphocyte subsets in kidney transplant recipients complicated by severe cytomegalovirus infection

目的 :探讨外周血 T淋巴细胞亚群的动态变化与肾移植受者并发重症巨细胞病毒 (CMV)感染之间的关系。方法 :采用 COUL TER公司鼠抗人 T淋巴细胞亚群单克隆抗体 OKT系列及 EL ITE型流式细胞仪 ,测定肾移植术后稳定、并发重症 CMV感染或急性排斥患者外周血 T淋巴细胞亚群。结果 :术后无急性排斥组与急性排斥组 ,其 CD4/CD8比值变化差异显著 (P<0 .0 5 ) ;而发生重症 CMV感染者 CD4/CD8比值变化与排斥者完全相反 ,且较长时间内维持在较低水平。结论 :外周血 T淋巴细胞亚群的动态变化对肾移植受者急性排斥和重症 CMV感染的诊断、鉴别诊断以及指导合理用药有一定意义。

Objective:To investigate the relationship between the clinical significance of the dynamic changes in T lymphocyte subsets of peripheral blood and the incidence and the severity of cytomegalovirus(CMV) infection in transplantation patients.Methods:T lymphocyte subsets of peripheral blood were consecutively assayed with miceversehuman Tlymphocyte subsets monoclonal antibodyOKT serials and flow cytometry.Results:The difference of CD 4/CD 8 ratios between the non acute rejection group and the acute rejection group was significantly different.In contrast to acute rejection,patients with severe CMV infection,the ratio was more persistently lower.Conclusions:These results indicate that monitoring of peripheral blood T lymphocyte subsets is valuable for the early diagnosis and the establishment of therapeutic regimen in kidney transplant recipients with acute rejection and(or) sever CMV infection.