红花性状标记杂交棉新品种鲁05H9 SSR指纹图谱构建及应用

Construction and application of SSR fingerprints of hybrid cotton variety Lu 05H9 with red-corolla marker trait

利用SSR分子标记技术对红花标记杂交棉新品种鲁05H9及其亲本材料进行多态性检测,从多态性高、重复性好的98对SSR核心引物中筛选出12对在双亲间具有多态性的引物、在F1中表现为杂合带的共显性标记。利用该12对引物构建了鲁05H9及其亲本的SSR指纹图谱。利用核心引物组合扩增的方法,可以鉴定鲁05H9的真实性和纯度,对于品种保护、纯度鉴定及杂交种推广具有极其重要的意义。

SSR markers were used to analyze the fingerprinting of hybrid cotton variety Lu 05H9 with red-corolla mark- er trait. Lu 05H9 and its parents were amplified with 98 pairs of core primers with high polymorphisms and good repeat- ability. Out of them, 12 paris were detected to be polymorphic between the two parents, which were codominant mark- ers since they amplified the different fragments between the two parents and heterozygosis bands in F1. Fingerprints of Lu05H9 were constructed with the amplification result of the 12 paris primers. It provides a method for seed genuineness and purity detection of hybrids. Due to its fast readability and large storage capacity, DNA fingerprinting of Lu05H9 is in favor of commercial applications.