穴位注射不同补泻手法对心气虚证模型大鼠心功能的影响

Effects of Point Injection with Different Reinforcing and Reducing Manipulation to Cardiac Function of Heart-qi Deficiency Syndrome Model in Rats

[目的]观察不同补泻手法的穴位注射对心气虚证模型大鼠心功能和血清SOD的影响。[方法]采用控食、负重强迫游泳及灌服大剂量心得安溶液等复合因素建立SD大鼠心气虚证模型。动物随机分为空白对照组(空白组)、模型对照组(模型组)、足三里穴注补法组(穴补组)、足三里穴注泻法组(穴泻组)。治疗组每穴注射参芪扶正注射液0.20ml,每日1次,连续治疗10天。治疗后,测定心功能相关血流动力学指标及血清SOD活性值。[结果](1)模型组大鼠血清SOD活性明显低于空白组(P<0.01);治疗10天后两治疗组与模型组相比差异有统计学意义(P<0.05或P<0.01);穴补组的血清SOD活性值大于穴泻组(P<0.01)。(2)模型组大鼠的心功能明显下降,两治疗组的心功能相关指标相应地出现了明显的变化和差异。穴补组的最大心室内压、左心室收缩压、+dp/dtmax、平均心室内压、心率、-dp/dtmax等指标均大于穴泻组的相应指标值(P<0.01)。[结论]不同补泻手法的穴位注射对疾病的治疗都具有影响,足三里穴注补法治疗心气虚证模型大鼠的效应明显优于足三里穴注泻法。

[Objective] To observe the influence of rats serum SOD and cardiac function with qi deficiency syndrome through the factors of the acupuncture point injection with different reinforcing and reducing methods. [Methods] To establish SD rat heart-qi deficiency syndrome model with the compound factors of the feeding, load forced swimming and large dose propranolol. The rats were randomly divided into blank control group, model group, Zusanli（St. 36） Shenqi Fuzheng injection reinforcing method group, Zusanli（St. 36） Shenqi Fuzheng injection reducing method group. Each acupoint injection of 0.20ml, one time a day, for 10 consecutive days. Determine of serum SOD activity values respectively and detect cardiac function correlated hemodynamic indexes. [Results] （1） The activity of serum SOD in model group rats was significantly lower than control group（ P〈0.01）;Treated groups and model group had significant difference（P〈0.05 or P＆lt;0.01）;Zusanli（St. 36） injection reinforcing method group and Zusanli（St. 36） reducing method group had significant differences（P〈0.01）;（2） The cardiac function of rats in model group decreased significantly, corresponding heart function index of each treatment groups had changes and obvious differences. The maximum ventricular rats model of the internal pressure, left ventricular systolic pressure ,the maximum rate of rise of left ventricular pressure, heart rate and other indicators of Zusanli（St. 36） Shenqi Fuzheng injection reinforcing method group were more than indexes of Zusanli（St. 36） Shenqi Fuzheng injection reducing method group（ P〈0.01）. [Conclusion] Acupoint injection of different reinforcing-reducing manipulations had different effect on the treatment of disease, and Zusanli（St. 36） injection reinforcement treatment of heart-qi deficiency syndrome model rats was significantly better than the other groups.