胡黄连苷Ⅱ对支气管哮喘大鼠气道炎症和支气管收缩影响的研究

Experimental study of picroside Ⅱ on airway inflammation and bronchial contraction in a rat asthmatic model

目的观察胡黄连苷Ⅱ对支气管哮喘（简称哮喘）大鼠气道炎症和支气管收缩的影响。方法将30只Wistar大鼠按随机数字表分成正常对照组（A组）、哮喘模型组（B组）、胡黄连苷Ⅱ干预组（c组），用卵白蛋白系统致敏加局部激发的方法制作哮喘模型，C组第8天起给予胡黄连苷II（100mg／kg）灌胃。第15天给予2oA卵白蛋白液诱喘，观察各组大鼠的诱喘表现、诱喘潜伏期变化。第22天给予气道阻力测定；BALF中细胞计数及分类计数、外周血嗜酸粒细胞（EOS）计数、酶联免疫吸附法测定血清和BALF中Th1／Th2细胞因子IFN-γ、IL-4水平；HE染色病理观察支气管肺组织切片。结果与B组相比，C组大鼠诱喘潜伏期明显延长（t=-5．961，P〈0．01），诱喘反应减轻；基础呼气阻力降低（t=11．014，P〈O．05），肺顺应性升高（t=-2．365，P〈O．05）；BALF中细胞总数和E0s计数明显减少（t值分别为16．685、5．519，P〈0．01）；外周血EOS计数显著降低（t=13．730，P〈O．01）；血清及BAI。F上清中IFN—Jy的水平显著升高（t值分别为-11．589、-9．177，P〈0．01），IL-4的水平显著降低（f值分别为13．728、25．649，P〈O．01）；支气管壁炎性细胞浸润减少，支气管壁及血管周围EOS浸润明显减少。结论胡黄连苷Ⅱ对哮喘大鼠具有一定的抑制支气管收缩的作用，并可以抑制哮喘大鼠气道局部炎症反应，改善气道局部和全身的Th亚群失衡。

Objective To observe picroside H effects on airway inflammation and bronchial contraction in a rat asthmatic model. Methods Thirty Wistar rats were randomly divided into three groups：control group （group A, n = 10）, asthmatic model group （group B, n = 10）, and picroside H treatment group（group C, n： 10）. Both group B and group C were sensitized by intraperitoneal injection of OVA and challenged by aerosolized OVA to establish the asthmatic model. Then the following examinations were performed on all rats. Provocation symptom and wheezing-induced delitescence were observed, the pulmonary function was tested, total cell number and differential inflammatory cell number in BALF and E（）S number in blood were counted under light microscope^the expressions of Thl cytokine （IFN-y） and Th2 cytokine （IL-4） of BALF and serum were quantified by ELISA technique; and the pathomorphological changes in tile lung tissue stained with HE were observed. Results Compared with group B, group C had a longer wheezing-induced delitescence, a lower expiratory resistance, a higher dynamic lung compliance, and a significant fewer number of WBC and EOS in BALF and in blood, an increased IFN-γ level and a decreased IL-4 level of BALF and serum, an alleviative bronchial inflammation. Conclusions Picroside Ⅱ can inhibit airway inflammation and bronchial contraction in rat asthmatic model, and can regulate the balance of Th2/Th2.